Efficient insertion of odd-numbered transmembrane segments of the tetracycline resistance protein requires even-numbered segments.

The Journal of Biological Chemistry
D GuoK W Miller

Abstract

Functional membrane insertion elements in the pBR322 tetracycline resistance protein were identified by comparing the ability of odd-numbered transmembrane segments and their attached periplasmic loops to insert into the membrane individually or when combined with the next even-numbered segment in the tetracycline resistance protein sequence. The efficiency with which individual odd-numbered segments and periplasmic loops inserted was probed by treating proteins truncated at the distal ends of periplasmic loops P2-P6 with carboxypeptidases and endoproteases in inside-out membrane vesicles. Insertion of odd-numbered segments and attached loops is inefficient when they occupy a C-terminal position in the protein. The C-terminal odd-numbered segment and loop sequences of 34-54% of the molecules of periplasmic loop truncation mutants could be removed by carboxypeptidase Y. In contrast, odd-numbered segments and loops insert efficiently if the next even-numbered segment in the sequence is present. In such cytoplasmic loop truncation mutants, only the cytoplasmic tail sequences of the proteins could be removed by carboxypeptidases. Remarkably, insertion of individual odd-numbered segments and loops is inefficient even though free ene...Continue Reading

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Citations

Jun 25, 2003·The Journal of Membrane Biology·R J Turner
Jun 30, 2000·Current Opinion in Cell Biology·R E DalbeyJ C Samuelson
Jun 6, 2009·Annual Review of Biochemistry·William Dowhan, Mikhail Bogdanov
May 28, 2003·Biochemical and Biophysical Research Communications·G Shane LewisKurt W Miller
Dec 18, 2013·Biochimica Et Biophysica Acta·Mikhail BogdanovHeidi Vitrac
Nov 18, 2004·Biochimica Et Biophysica Acta·Ross E Dalbey, Minyong Chen
Jan 7, 2004·The Journal of Biological Chemistry·Yoko DohkeR James Turner
Jul 31, 2002·Archives of Biochemistry and Biophysics·G Shane LewisKurt W Miller

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