PMID: 9551603Apr 29, 1998Paper

Efficient, interactive, and three-dimensional segmentation of cell nuclei in thick tissue sections

Cytometry
S LockettJ W Gray

Abstract

Segmentation of intact cell nuclei in three-dimensional (3D) images of thick tissue sections is an important basic capability necessary for many biological research studies. Because automatic algorithms do not correctly segment all nuclei in tissue sections, interactive algorithms may be preferable for some applications. Existing interactive segmentation algorithms require the analyst to draw a border around the nucleus under consideration in all successive two-dimensional (2D) planes of the 3D image. The present paper describes an algorithm with two main advantages over the existing method. First, the analyst draws borders only in 2D planes that cut approximately through the center of the nucleus under consideration so that the nuclear borders generally are most distinct. Second, the analyst draws only five borders around each nucleus, and then the algorithm interpolates the entire surface. The algorithm results in segmented objects that correspond to individual, visually identifiable nuclei. The segmented surfaces, however, may not exactly represent the true nuclear surface. An optional, automatic surface optimization algorithm can be applied to reduce this error.

Citations

Sep 29, 2009·Computer Methods and Programs in Biomedicine·Matteo CampanaAlessandro Sarti
Mar 3, 2007·Computers in Biology and Medicine·Hyun-Ju Choi, Heung-Kook Choi
Mar 18, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·David SvobodaStanislav Stejskal
Oct 21, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Gang LinBadrinath Roysam
Oct 12, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ki Hean KimPeter T C So
Feb 7, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Rachid BenchaouirCorinne Laplace-Builhé
Jun 17, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Robert F Murphy
Apr 10, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Cheng ChenGustavo K Rohde
Jan 13, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Patrik MalmEwert Bengtsson
Dec 4, 2009·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Cecilia ZanellaAlessandro Sarti
Jul 26, 2007·IEEE Transactions on Medical Imaging·Antti LehmussolaOlli Yli-Harja
Aug 4, 2004·Micron : the International Research and Review Journal for Microscopy·Noël Bonnet
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Aug 11, 2006·Current Opinion in Biotechnology·Rodrigo Fernández-GonzálezCarlos Ortiz-de-Solorzano
Jun 28, 2016·BMC Bioinformatics·Violeta N KovachevaNasir M Rajpoot
Dec 7, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Vladimír UlmanPekka Ruusuvuori
Sep 14, 2016·IEEE Transactions on Medical Imaging·David Svoboda, Vladimir Ulman
Apr 20, 2005·Journal of Mammary Gland Biology and Neoplasia·Rodrigo Fernandez-GonzalezCarlos Ortiz-de-Solórzano
Sep 5, 2008·Current Protocols in Cytometry·S J Lockett
Mar 17, 2019·BMC Bioinformatics·Bastien KovacPierre Weiss

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