Efficient isolation and sensitive quantification of extracellular vesicles based on an integrated ExoID-Chip using photonic crystals

Lab on a Chip
Xing DongHong Liu

Abstract

Extracellular vesicles (EVs), involved in many diseases and pathophysiological processes, have emerged as potential biomarkers for cancer diagnosis. However, efficient isolation and detection of EVs still remain challenging. Here, we report an integrated chip for isolation of EVs with a double-filtration unit and ultrasensitive detection using photonic crystal (PC) nanostructure. Nanofiltration membranes were integrated into the device to isolate and enrich the EVs of 20-200 nm in size based on size-exclusion. Then, CD63 aptamers were used to combine the EVs on the nanofiltration membrane with a pore size of 20 nm, and excess aptamers passed through the membrane to bind with CD63 immobilized on the PC nanostructure. Benefitting from the fluorescence enhancement effect of the PC nanostructure in competition assays, the EVs could be quantified sensitively by analyzing the concentration of excess aptamers. Due to the high sensitivity, the limit of detection was as low as 8.9 × 103 EVs per mL with a low sample consumption of only 20 μL. Furthermore, serum samples from breast cancer patients and healthy donors could be successfully distinguished. Thus, this microfluidic chip provides an effective method for pre-screening of cancer i...Continue Reading

References

Jun 6, 2009·Nature Reviews. Immunology·Clotilde ThéryElodie Segura
Feb 4, 2010·Lab on a Chip·Chihchen ChenDaniel Irimia
Aug 10, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Sandrine RoriveIsabelle Salmon
Feb 2, 2011·Journal of Colloid and Interface Science·Heng LiRongming Wang
Aug 28, 2012·Advanced Drug Delivery Reviews·Atsushi SuetsuguRobert M Hoffman
Nov 1, 2012·Lab on a Chip·Ryan T DaviesJaesung Park
Sep 7, 2013·Journal of Extracellular Vesicles·Yusuke YoshiokaTakahiro Ochiya
Jun 24, 2014·Clinical Biochemistry·Josselin CaradecEmma Guns
Feb 13, 2015·ACS Nano·Kyungheon LeeHakho Lee
Jan 26, 2016·ACS Nano·Sangmoo JeongHakho Lee
Apr 14, 2016·Scientific Reports·Mark A RiderDavid G Meckes
Jun 18, 2017·Analytical Chemistry·Junjie ChiZhongze Gu
Nov 2, 2017·ACS Nano·Fei LiuUtkan Demirci
Feb 28, 2018·Electrophoresis·Steven MarczakHsueh-Chia Chang
Jun 12, 2018·Nano Letters·Chunchen LiuLei Zheng
Oct 20, 2018·ACS Applied Materials & Interfaces·Junjie ChiZhongze Gu
May 28, 2019·Nature Biomedical Engineering·Peng ZhangYong Zeng

❮ Previous
Next ❯

Citations

Feb 8, 2020·Biotechnology Journal·Guiying LiFang Yang
Aug 12, 2020·Cancer Medicine·Jing Wu, Zuojun Shen
Aug 28, 2020·Lab on a Chip·Yang LiuLeidong Mao
Feb 27, 2021·Analytical Chemistry·He YanYong Zeng
Feb 26, 2020·Biosensors & Bioelectronics·Su WangZhiyang Li
Mar 6, 2021·Chemical Reviews·Lingling WuWeihong Tan
Jan 3, 2020·ACS Applied Materials & Interfaces·Shilian DongXiangheng Xiao
Sep 1, 2021·Advanced Science·Li MinShutao Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
pull-down
chip
Bragg diffraction
Fluorescence

Software Mentioned

SPSS Statistics
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Visualized Experiments : JoVE
Kaori IshiguroTushar Patel
American Journal of Physiology. Cell Physiology
Fumihiko UrabeTakahiro Ochiya
Circulation Research
Frank A W CoumansRienk Nieuwland
© 2021 Meta ULC. All rights reserved