Efficient isolation of human parainfluenza viruses 1 and 3 using MNT-1, a human malignant melanoma cell line system that exhibits an apparent cytopathic effect

Microbiology and Immunology
Ko SatoH Nishimura

Abstract

Isolation of human parainfluenza virus (HPIV) serotypes 1 and 3 from clinical specimens is not very efficient because of the lack of a cell culture system capable of inducing CPE. In this study, the utility of a melanoma cell line, MNT-1, that allows HPIV growth and displays CPE was demonstrated. In particularly, the efficiency of isolating HPIV1 and HPIV3 using MNT-1 was greater than for cell lines conventionally used for HPIV isolation. Our demonstrated efficacy of HPIV1 and HPIV3 isolation with apparent CPE using the MNT-1 cell culture system has the potential to improve virus isolation from clinical specimens.

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Related Concepts

Cytopathogenic Effect, Viral
Melanoma
Para-Influenza Virus Type 1
Para-Influenza Virus Type 3
Paramyxoviridae Infections
Cell Culture Techniques
Cell Line, Tumor
Cell Culture Techniques
Cytopathogenic Effect, Viral
Melanoma

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