PMID: 3763395Sep 11, 1986Paper

Efficient isolation of the linear DNA killer plasmid of Kluyveromyces lactis: evidence for location and expression in the cytoplasm and characterization of their terminally bound proteins

Nucleic Acids Research
J C StamA R Stuitje

Abstract

Differential centrifugation of an osmotic lysate of K. lactis protoplasts showed that the linear DNA killer plasmids of K. lactis, pGKL1 and pGKL2, are almost exclusively present in the cytoplasmic fraction. This fractionation procedure allows the rapid isolation of large amounts of plasmid DNA without contamination by chromosomal and mitochondrial DNA. With these DNA preparations the size of the terminally bound proteins was estimated to be 28 and 36 kDal for pGKL1 and pGKL2, respectively. The entire pGKL1 sequence (except for 21 base pairs at the right terminus) was cloned in a shuttle vector that permits autonomous replication in the nucleus of K. lactis. However, killer gene expression could not be established in transformants. In connection with the observed cytoplasmic localization, this result suggests that gene expression of the killer DNA plasmids is entirely cytoplasmic.

References

Nov 1, 1991·Molecular & General Genetics : MGG·M Ohman, E G Wagner
Oct 11, 1984·Nucleic Acids Research·F HishinumaT Maeda
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K WatabeJ Ito
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Citations

Apr 1, 1991·Molecular & General Genetics : MGG·F Hishinuma, K Hirai
Feb 1, 1990·Current Genetics·F MeinhardtK Esser
Feb 1, 1992·Current Genetics·H S Shepherd
May 1, 1994·Current Genetics·S H Jabaji-HareB F Lang
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D G McNeel, F Tamanoi
Jul 11, 1988·Nucleic Acids Research·M TommasinoC L Galeotti
Dec 1, 2000·Applied and Environmental Microbiology·W B ChenS C Chang
Apr 17, 2016·Journal of Biotechnology·Lukas SturmbergerAnton Glieder
Aug 15, 1995·FEMS Microbiology Letters·H Fukuhara
Aug 17, 2014·Applied and Environmental Microbiology·Sabrina WemhoffFriedhelm Meinhardt
Dec 1, 1988·European Journal of Epidemiology·N Gunge, K Kitada
Dec 24, 2018·Biochemical Society Transactions·David Forrest
Sep 1, 1989·Microbiological Reviews·F C VolkertJ R Broach

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