Efficient method for rapid induction of aberrant crypt foci in rats with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine

Japanese Journal of Cancer Research : Gann
M OchiaiM Nagao

Abstract

2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) induces aberrant crypt foci (ACFs) as well as colon cancer in F344 male rats. Conditions allowing rapid development of ACFs over a short period were investigated. F344 male rats were fed 400 ppm of PhIP x HCl in a low-fat diet (LF) for 2 weeks and then given a PhIP-free, high-fat diet containing PRIMEX (HF-PR) or safflower oil, or PhIP-free LF for 4 or 12 weeks. Rats fed HF-PR for 4 weeks gave the highest number of ACFs/rat (3.3) and their size in terms of aberrant crypts/ACF (2.7) was much larger than that obtained with conventional continuous feeding of PhIP for 25 weeks in the CE-2 diet. Therefore, 2 weeks of dietary exposure to 400 ppm of PhIP x HCl, followed by HF-PR for 4 weeks, is a practical and convenient method for obtaining ACFs. This protocol should be useful for studies of the early phase of colon carcinogenesis.

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Citations

Sep 28, 2002·Mutation Research·Hitoshi NakagamaMinako Nagao
Jul 24, 1998·Mutation Research·M NagaoH Nakagama
May 16, 2014·Cancer Science·Masako OchiaiHitoshi Nakagama
Oct 20, 2005·Cancer Science·Hitoshi NakagamaMasako Ochiai
Jun 9, 2007·International Journal of Cancer. Journal International Du Cancer·Hiroyuki KohnoTakuji Tanaka
Jan 15, 2019·Cancer Science·Yoshiaki MaruYoshitaka Hippo
Jun 22, 1999·British Journal of Cancer·S K PandaS A Broitman
Feb 11, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shivangi TrivediPatricia L Brubaker

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