PMID: 9188837May 9, 1997Paper

Efficient method for the quantitation of urinary leukotriene E4: extraction using an Empore C18 disk cartridge

Journal of Chromatography. B, Biomedical Sciences and Applications
Haruhisa MitaKazuo Akiyama

Abstract

We describe here an efficient procedure for the precise quantitation of leukotriene E4 (LTE4) in a small volume of urine, which was achieved mainly by the use of an Empore extraction disk cartridge. After addition of [3H]LTE4 to 2 ml of urine, an Empore C18 cartridge was used for initial extraction of the urine, which resulted in the extraction of LTE4 in a small volume of solvent. The eluate could then be injected onto a high-performance liquid chromatography column without further concentration. After separation by high-performance liquid chromatography, LTE4 was extracted from the effluent using an Empore C18 cartridge. The concentration of LTE4 was subsequently quantified by enzyme immunoassay. LTE4 can be recovered from urine with sufficient efficiency (69.9+/-4.7%, mean+/-S.D., n=101). The coefficient of variation of the assay procedure was less than 10%. When urine was spiked with different amounts of LTE4, the recovery of LTE4 added to the urine specimen was less than 120%. The concentration of LTE4 in urine from normal healthy subjects was 48.0+/-15.3 pg/mg creatinine (n=15).

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Citations

Sep 23, 2003·American Journal of Respiratory and Critical Care Medicine·F Runa AliA Barry Kay
Oct 10, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·N HigashiK Akiyama
Jul 22, 2015·The Journal of Allergy and Clinical Immunology·Chihiro MitsuiMasami Taniguchi
Jul 16, 2008·The Journal of Allergy and Clinical Immunology·Emiko OnoKazuo Akiyama
Aug 10, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H MitaK Akiyama
May 15, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E OnoK Akiyama
Jan 9, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E OnoK Akiyama
Nov 19, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H YamaguchiM Taniguchi
Nov 26, 2010·Allergology International : Official Journal of the Japanese Society of Allergology·Emiko OnoKazuo Akiyama
Oct 24, 2008·Allergology International : Official Journal of the Japanese Society of Allergology·Masami TaniguchiKazuo Akiyama
Jan 30, 2007·Prostaglandins & Other Lipid Mediators·Haruhisa MitaMasami Taniguchi
Apr 30, 2017·The Journal of Allergy and Clinical Immunology·Chihiro MitsuiMasami Taniguchi
Oct 11, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Melanie Markmann, Diethard Tautz
Jan 14, 2018·Applied and Environmental Microbiology·C BerginN Dubilier
May 26, 2012·Allergology International : Official Journal of the Japanese Society of Allergology·Noritaka HigashiKazuo Akiyama

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