Efficient mining of myxobacterial metabolite profiles enabled by liquid chromatography-electrospray ionisation-time-of-flight mass spectrometry and compound-based principal component analysis

Analytica Chimica Acta
Daniel KrugRolf Müller

Abstract

Bacteria producing secondary metabolites are an important source of natural products with highly diverse structures and biological activities. Developing methods to efficiently mine procaryotic secondary metabolomes for the presence of potentially novel natural products is therefore of considerable interest. Modern mass spectrometry-coupled liquid chromatography can effectively capture microbial metabolic diversity with ever improving sensitivity and accuracy. In addition, computational and statistical tools increasingly enable the targeted analysis and exploration of information-rich LC-MS datasets. In this article, we describe the use of such techniques for the characterization of myxobacterial secondary metabolomes. Using accurate mass data from high-resolution ESI-TOF measurements, target screening has facilitated the rapid identification of known myxobacterial metabolites in extracts from nine Myxococcus species. Furthermore, principal component analysis (PCA), implementing an advanced compound-based bucketing approach, readily revealed the presence of further compounds which contribute to variation among the metabolite profiles under investigation. The generation of molecular formulae for putative novel compounds with hig...Continue Reading

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Citations

Oct 15, 2013·International Journal of Medical Microbiology : IJMM·Rolf Müller, Joachim Wink
Dec 18, 2013·Journal of Industrial Microbiology & Biotechnology·Brian O BachmannRichard H Baltz
Jun 21, 2011·Current Opinion in Chemical Biology·Esther K SchmittFrank Petersen
Jul 22, 2009·Phytochemistry·Holger Jenke-Kodama, Elke Dittmann
Jan 27, 2012·Chembiochem : a European Journal of Chemical Biology·Dominik Pistorius, Rolf Müller
Jul 25, 2009·Biomedical Chromatography : BMC·T Cserháti
Sep 10, 2014·Angewandte Chemie·Thomas P WycheTim S Bugni
Jul 21, 2015·Drug Discovery Today. Technologies·Changsheng WuYoung Hae Choi
May 23, 2009·Molecular BioSystems·Silke C Wenzel, Rolf Müller
Oct 22, 2009·Natural Product Reports·Silke C Wenzel, Rolf Müller
Jun 4, 2010·Natural Product Reports·Kira J Weissman, Rolf Müller
Dec 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rafidah AhmadSyarul Nataqain Baharum
May 9, 2009·Chemical Reviews·Gordon M CraggDavid J Newman
May 21, 2010·Analytical Chemistry·Barry Lavine, Jerry Workman
Sep 19, 2012·Organic Letters·Yanpeng HouTim S Bugni
Apr 19, 2019·ACS Synthetic Biology·Domen PogorevcSilke C Wenzel

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