Efficient monocyte-derived dendritic cell generation in patients with acute myeloid leukemia after chemotherapy treatment: application to active immunotherapy

Experimental Hematology
Pierre-Joseph RoyerMarc Gregoire

Abstract

While complete remission in acute myeloid leukemia (AML) can be achieved after chemotherapy (CT), relapses occur for the majority of patients, underlying the need to eliminate residual disease. Based on dendritic cell (DC) vaccination, the triggering of an immune response against residual leukemia cells after CT could maintain patients in remission. The aim of our study was to assess, for vaccine preparation, generation of monocyte-derived DCs in AML patients after CT. We evaluated efficiency of the production, yields, maturation, and functional properties of DCs from 22 AML patients at different CT stages compared to those from 15 healthy donors. We demonstrated that monocyte-derived DC production is successful later than 3 weeks after the last CT cycle, whatever the CT was. Immature DCs demonstrated functional phagocytic activity. Mature DCs displayed migratory, T-cell stimulatory and Th1-activation capacities. Our results also suggest a favorable period from 20 to 60 days after CT for potent monocyte-derived DC production and immune activation. In defining patient-sampling conditions, this preclinical study has direct implications for AML DC-based immunotherapy.

References

Aug 3, 2000·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·D M Lang
Apr 26, 2002·British Journal of Haematology·Qing YiNikhil Munshi
Sep 7, 2002·Journal of Immunological Methods·Thomas G BergerBeatrice Schuler-Thurner
Mar 6, 2003·The Journal of Clinical Investigation·Jeffrey J MolldremMark M Davis
Nov 25, 2003·Hematology·Bob LöwenbergMartin S Tallman
Jan 23, 2004·Cancer Investigation·Derry Ridgway
May 4, 2004·Nature Medicine·Carl G FigdorCornelis J M Melief
Jun 9, 2004·Critical Reviews in Oncology/hematology·Matthew SmithWolfgang Kern
Apr 26, 2005·Journal of Immunological Methods·Thomas G BergerGerold Schuler
Jun 13, 2006·Scandinavian Journal of Immunology·P-J RoyerM Gregoire
Nov 6, 2007·Immunological Reviews·Shin-Ichiro FujiiRalph M Steinman

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Citations

May 13, 2014·Immunotherapy·Dige Andom Tesfatsion
Sep 16, 2008·Best Practice & Research. Clinical Haematology·Caroline Duncan, Huw Roddie
May 13, 2008·Leukemia Research·Willemijn van den AnckerArjan A van de Loosdrecht
Jun 13, 2012·Cytotherapy·Sébastien AnguilleZwi N Berneman
Oct 1, 2008·Hematology/oncology and Stem Cell Therapy·Celso MassumotoLuiz H Camara-Lopes
Jan 19, 2016·Cell Transplantation·Mohammad HaqueJianxun Song

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