Efficient Plant Production of Recombinant NS1 Protein for Diagnosis of Dengue

Frontiers in Plant Science
Mariana F XistoSérgio O De Paula

Abstract

Dengue fever is endemic in more than 120 countries, which account for 3.9 billion people at risk of infection worldwide. The absence of a vaccine with effective protection against the four serotypes of this virus makes differential molecular diagnosis the key step for the correct treatment of the disease. Rapid and efficient diagnosis prevents progression to a more severe stage of this disease. Currently, the limiting factor in the manufacture of dengue (DENV) diagnostic kits is the lack of large-scale production of the non-structural 1 (NS1) protein (antigen) to be used in the capture of antibodies from the blood serum of infected patients. In this work, we use plant biotechnology and genetic engineering as tools for the study of protein production for research and commercial purposes. Gene transfer, integration and expression in plants is a valid strategy for obtaining large-scale and low-cost heterologous protein production. The authors produced NS1 protein of the dengue virus serotype 2 (NS1DENV2) in the Arabidopsis thaliana plant. Transgenic plants obtained by genetic transformation expressed the recombinant protein that was purified and characterized for diagnostic use. The yield was 203 μg of the recombinant protein per ...Continue Reading

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Citations

Aug 7, 2021·Vaccines·Srividhya VenkataramanMounir Abouhaidar

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Datasets Mentioned

BETA
MK506264

Methods Mentioned

BETA
glycosylation
ELISA
transgenic
transfections
enzyme-linked immunosorbent assays
electrophoresis
dot-blot
confocal microscopy
Dot
transfection

Software Mentioned

GraphPad Prism
ImageJ
GraphPad

Related Concepts

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