PMID: 9185578Jul 1, 1997Paper

Efficient priming of PCR with short oligonucleotides conjugated to a minor groove binder

Nucleic Acids Research
I AfoninaR B Meyer

Abstract

The tripeptide 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3) binds to the minor groove of DNA with high affinity. When this minor groove binder (MGB) is conjugated to the 5'-end of short oligodeoxynucleotides (ODNs), the conjugates form unusually stable hybrids with complementary DNA in which the tethered CDPI3group resides in the minor groove. We show that these conjugates can be used as PCR primers. Due to their unusually high binding affinity, conjugates as short as 8-10mers can be used to amplify DNA with good specificity and efficiency. The reduced length primers described here might be appropriate for the PCR amplification of viral sequences which possess a high degree of variability (e.g., HPV, HIV) or for recent techniques such as gene hunting and differential display which amplify multiple sequences using short primer pairs.

References

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Citations

Jan 18, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Romulo Martin BrenaChristoph Plass
Jun 17, 2011·Journal of Genetics·Mohamed Z GadKhaled Abou-Aisha
Nov 7, 2003·Molecular and Cellular Probes·Capucine LetertrePatrick Fach
Feb 1, 2005·The Journal of Molecular Diagnostics : JMD·Michihiro UchiyamaTomoyuki Masuda
Feb 26, 2010·Nucleosides, Nucleotides & Nucleic Acids·Naoki ShinodaKatsuaki Sugiura
Mar 14, 1998·Nucleic Acids Research·S KumarB I Schweitzer
May 25, 2010·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·S KlimpeA U Mannan
Mar 18, 2004·Journal of Neurology, Neurosurgery, and Psychiatry·K E WilsonC M Morris
Jun 29, 2007·Annals of Surgical Oncology·Tari A KingJeff Boyd
Dec 25, 2013·PloS One·Joel González-CabreraMartin S Williamson
Apr 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sarah J BearchellBruce D L Fitt
Feb 19, 2014·International Journal of Food Microbiology·Lourdes Soto-MuñozRosario Torres
Jan 6, 2011·Journal of Pediatric Gastroenterology and Nutrition·Adrienne LeeKevin P Rioux
Apr 5, 2002·Human Mutation·Pui-Yan Kwok
Sep 13, 2008·Expert Review of Molecular Diagnostics·François RouetEric Nerrienet
Oct 13, 2010·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Mohamed Z GadHossam A Elgabarty
Dec 3, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·E NavarroJ Solera

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