Efficient Production of 2,5-Diketo-d-Gluconate via Heterologous Expression of 2-Ketogluconate Dehydrogenase in Gluconobacter japonicus

Applied and Environmental Microbiology
Naoya KataokaK Matsushita

Abstract

2,5-Diketo-d-gluconate (2,5DKG) is a compound that can be the intermediate for d-tartrate and also vitamin C production. Although Gluconobacter oxydans NBRC3293 produces 2,5DKG from d-glucose via d-gluconate and 2-keto-d-gluconate (2KG), with accumulation of the product in the culture medium, the efficiency of 2,5DKG production is unsatisfactory because there is a large amount of residual d-gluconate at the end of the biotransformation process. Oxidation of 2KG to 2,5DKG is catalyzed by a membrane-bound flavoprotein-cytochrome c complex: 2-keto-gluconate dehydrogenase (2KGDH). Here, we studied the kgdSLC genes encoding 2KGDH in G. oxydans NBRC3293 to improve 2,5DKG production by Gluconobacter spp. The kgdS, kgdL, and kgdC genes correspond to the small, large, and cytochrome subunits of 2KGDH, respectively. The kgdSLC genes were cloned into a broad-host-range vector carrying a DNA fragment of the putative promoter region of the membrane-bound alcohol dehydrogenase gene of G. oxydans for expression in Gluconobacter spp. According to our results, 2KGDH that was purified from the recombinant Gluconobacter cells showed characteristics nearly the same as those reported previously. We also expressed the kgdSLC genes in a mutant strain...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 11, 1986·Nucleic Acids Research·G von Heijne
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jan 1, 1994·Advances in Microbial Physiology·K MatsushitaO Adachi
Mar 1, 1993·Microbiological Reviews·A P Pugsley
Aug 22, 2001·Protein Science : a Publication of the Protein Society·O Dym, D Eisenberg
Feb 28, 2002·Bioscience, Biotechnology, and Biochemistry·Teruhide Sugisawa, Tatsuo Hoshino
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Apr 2, 2005·Current Opinion in Microbiology·Ben C BerksFrank Sargent
May 1, 1982·Applied and Environmental Microbiology·T SonoyamaK Mitsushima
Mar 7, 2006·Journal of Biotechnology·Christoph BremusHermann Sahm
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jan 9, 2009·Bioscience, Biotechnology, and Biochemistry·Emiko ShinagawaKazunobu Matsushita
Oct 20, 2009·Applied and Environmental Microbiology·Hiroshi HabeKeiji Sakaki
Dec 15, 2010·Bioscience, Biotechnology, and Biochemistry·Osao AdachiKazunobu Matsushita
Nov 22, 2013·Advances in Biochemical Engineering/biotechnology·Günter Pappenberger, Hans-Peter Hohmann

❮ Previous
Next ❯

Citations

Sep 4, 2019·Acta Crystallographica. Section D, Structural Biology·Hiromi YoshidaKoji Sode
Dec 28, 2017·Applied Microbiology and Biotechnology·Anna SiemenUwe Deppenmeier
Apr 16, 2021·Applied Microbiology and Biotechnology·Philipp Moritz FrickeTino Polen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.