Efficient production of erythroid, megakaryocytic and myeloid cells, using single cell-derived iPSC colony differentiation

Stem Cell Research
Marten HansenEmile van den Akker

Abstract

Hematopoietic differentiation of human induced pluripotent stem cells (iPSCs) provide opportunities not only for fundamental research and disease modelling/drug testing but also for large-scale production of blood effector cells for future clinical application. Although there are multiple ways to differentiate human iPSCs towards hematopoietic lineages, there is a need to develop reproducible and robust protocols. Here we introduce an efficient way to produce three major blood cell types using a standardized differentiation protocol that starts with a single hematopoietic initiation step. This system is feeder-free, avoids EB-formation, starts with a hematopoietic initiation step based on a novel single cell-derived iPSC colony differentiation and produces multi-potential progenitors within 8-10 days. Followed by lineage-specific growth factor supplementation these cells can be matured into well characterized erythroid, megakaryocytic and myeloid cells with high-purity, without transcription factor overexpression or any kind of pre-purification step. This standardized differentiation system provides a simple platform to produce specific blood cells in a reproducible manner for hematopoietic development studies, disease modellin...Continue Reading

Citations

Nov 8, 2019·Blood Advances·Steven HeshusiusEmile van den Akker
Jul 30, 2020·International Journal of Molecular Sciences·Cécile DeleschauxMariano A Ostuni
Jan 29, 2021·Development, Growth & Differentiation·Sou NakamuraKoji Eto
Aug 10, 2021·Frontiers in Immunology·Olga ZimmermannovaCarlos-Filipe Pereira

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