Efficient production of laccases by Trametes sp. AH28-2 in cocultivation with a Trichoderma strain

Applied Microbiology and Biotechnology
H ZhangX Q Zhang

Abstract

A biocontrol fungus isolated from rotting wood was identified as a Trichoderma strain (named as Trichoderma sp. ZH1) by internal transcribed spacer (ITS) sequences of rRNA genes. The laccase yield of Trametes sp. AH28-2 in cocultivation with Trichoderma sp. ZH1 reached 6,210 U l(-1), approximately identical to those induced by toxic aromatic inducers. Cocultures maintained 60-70 % of their highest laccase activity obtained at 5 days after inoculation of the biocontrol fungus, at least for 20 days. Furthermore, a novel laccase isozyme (LacC) was obtained through the fungal interactions. The molecular weight of LacC is about 64 kDa, and its isoelectric point is 6.6. The temperature and pH optimum for LacC to oxidize guaiacol are 55 degrees C and 5.0, respectively. LacC is stable both at 60 degrees C and pH 4.0-8.0. Furthermore, the K (m) values of LacC for various substrates were also determined. Our work demonstrates a safe strategy for the production of industrial laccases, instead of the traditional method of chemical induction.

References

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Citations

Jul 6, 2012·Bioprocess and Biosystems Engineering·Tuğba Aydinoğlu, Sayit Sargin
Jun 8, 2011·Applied Biochemistry and Biotechnology·Chen Qi-HeSusanne Zibek
Jun 13, 2013·Journal of Biochemistry·André Ricardo de Lima DamásioMaria de Lourdes T M Polizeli
Jun 30, 2009·Journal of Biotechnology·Vladimir Elisashvili, Eva Kachlishvili
Nov 10, 2015·Journal of Biotechnology·Jingjing WangYazhong Xiao
Jan 15, 2010·Journal of Applied Microbiology·J BaderU Stahl
May 10, 2015·Applied Microbiology and Biotechnology·N VassilevE Malusà
May 5, 2010·Enzyme and Microbial Technology·María I FonsecaLaura L Villalba
Jun 1, 2016·Frontiers in Microbiology·Lakshmanan Divya, C Sadasivan
Jun 1, 2016·Frontiers in Microbiology·Sujatha KandasamySivakumar Uthandi
Jan 19, 2010·Journal of Basic Microbiology·Fen WeiYazhong Xiao

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