Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli

Bioresource Technology
Bing BaiJian-min Xing

Abstract

In this study, microbial production of succinic acid from macroalgae (i.e., Laminaria japonica) was investigated for the first time. The engineered Escherichia coli BS002 exhibited higher molar yield of succinic acid on mannitol (1.39±0.01mol/mol) than glucose (1.01±0.05mol/mol). After pretreatment and enzymatic hydrolysis, L. japonica hydrolysate was mainly glucose (10.31±0.32g/L) and mannitol (10.12±0.17g/L), which was used as the substrate for succinic acid fermentation with the recombinant BS002. A final 17.44±0.54g/L succinic acid was obtained from the hydrolysate after 72h dual-phase fermentation. The yield was as high as 1.24±0.08mol/mol total sugar, which reached 73% of the maximum theoretical yield. The results demonstrate that macroalgae biomass represents a novelty and economical alternative feedstock for biochemicals production.

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Citations

Apr 2, 2019·Critical Reviews in Biotechnology·Mariateresa FeroneAntonio Marzocchella
Nov 11, 2016·Engineering in Life Sciences·Julian LangeBastian Blombach
Sep 28, 2018·Applied Microbiology and Biotechnology·Wubliker DessieMin Jiang
Jul 15, 2018·Bioprocess and Biosystems Engineering·Ayobami Matthew OlajuyinJianmin Xing
May 11, 2020·Trends in Biotechnology·Lieve M L LaurensRobert S Nelson

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