PMID: 9187252May 23, 1997Paper

Efficient purification, characterization and partial amino acid sequencing of two alpha-1,4-glucan lyases from fungi

Biochimica Et Biophysica Acta
S YuJ Marcussen

Abstract

alpha-1,4-Glucan lyases from the fungi Morchella costata and M. vulgaris were purified by affinity chromatography on beta-cyclodextrin-sepharose, followed by ion exchange and gel filtration. The purified enzymes produced 1,5-anhydro-D-fructose from glucose oligomers and polymers with alpha-1,4-glucosidic linkages, such as maltose, maltosaccharides, amylopectin, and glycogen. The lyases were basically inactive towards glucans linked through alpha-1,1, alpha-1,3 or alpha-1,6 linkages. For both enzymes the molecular mass was around 121,000 Da as determined by matrix-assisted laser desorption mass spectrometry. The pI for the lyases from M. costata and M. vulgaris was 4.5 and 4.4, respectively. The lyases exhibited an optimal pH range of pH 5.5 to pH 7.5 with maximal activity at pH 6.5. Optimal temperature was between 37 degrees C and 48 degrees C for the two lyases, depending on the substrates. The lyases were examined with 12 inhibitors to starch hydrolases and it was found that they were inhibited by the -SH group blocking agent PCMB and the following sugars and their analogues: glucose, maltitol, maltose, 1-deoxynojirimycin and acarbose. Partial amino acid sequences accounting for about 35% of the lyase polypeptides were determ...Continue Reading

Citations

Sep 18, 2002·Journal of Applied Microbiology·L V ThomasJ Delves-Broughton
Oct 27, 2001·Applied and Environmental Microbiology·A Da CostaJ Courtois
Sep 12, 2008·IUBMB Life·Shukun Yu
Jul 11, 2006·Biochimica Et Biophysica Acta·Shukun Yu, Roland Fiskesund
Aug 6, 2014·Bioscience, Biotechnology, and Biochemistry·Janjira ManeesanAtsuo Kimura
May 28, 2004·International Journal of Food Microbiology·Linda V ThomasJoss Delves-Broughton
Jul 1, 2004·Critical Reviews in Biotechnology·P MichaudJ Courtois
Aug 2, 2013·The Journal of Biological Chemistry·Henriëtte J RozeboomBauke W Dijkstra
May 15, 2002·Carbohydrate Research·Søren M AndersenShukun Yu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.