Efficient reproduction of cynomolgus monkey using pronuclear embryo transfer technique.

Proceedings of the National Academy of Sciences of the United States of America
Qiang SunJoe Z Tsien

Abstract

One of the technical bottlenecks in producing nonhuman primate models is that current assisted reproductive techniques, such as in vitro culture and frozen conservation of multicell-stage embryos, often result in poor embryo quality and subsequently lead to low birth rates. We investigated whether pronuclear embryo transfer can be used as an effective means for improving pregnancy and live birth rates of nonhuman primates. We collected 174 metaphase II oocytes by laparoscopy from 22 superovulated mature females and then fertilized these eggs using either in vitro fertilization or intracytoplasmic sperm injection, resulting in a 33.3% and a 50% fertilization rate, respectively. These 66 fertilized pronuclear-stage embryos were then tubally transferred to 30 recipients and led to 7 births and 1 abortion. Importantly, we observed that the highest live birth rate of approximately 64% was obtained when the transfer of pronuclear embryos was performed in the presence of new corpus luteum in the ovary of recipients between 24 h and 36 h after estradiol peak. Therefore, our experiments demonstrate that by matching the critical time window in the recipient's reproductive cycle for achieving optimal embryo-uterine synchrony, pronuclear e...Continue Reading

References

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Citations

Sep 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yuyu NiuWeizhi Ji
Jul 13, 2012·The Journal of Veterinary Medical Science·Kazuhiro NakamaHiromichi Tamada
May 20, 2014·Neuroscience Bulletin·Zhen LiuZilong Qiu
Jul 4, 2012·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yongchang ChenWeizhi Ji
Apr 19, 2012·American Journal of Primatology·Yongchang ChenWeizhi Ji
Jan 16, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Youjin HuGuoping Fan
Oct 30, 2020·Scientific Reports·Jenna Kropp SchmidtIgor I Slukvin

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