Efficient reverse transcription using locked nucleic acid nucleotides towards the evolution of nuclease resistant RNA aptamers.

PloS One
Lucile CrouzierRakesh N Veedu

Abstract

Modified nucleotides are increasingly being utilized in the de novo selection of aptamers for enhancing their drug-like character and abolishing the need for time consuming trial-and-error based post-selection modifications. Locked nucleic acid (LNA) is one of the most prominent and successful nucleic acid analogues because of its remarkable properties, and widely explored as building blocks in therapeutic oligonucleotides. Evolution of LNA-modified RNA aptamers requires an efficient reverse transcription method for PCR enrichment of the selected RNA aptamer candidates. Establishing this key step is a pre-requisite for performing LNA-modified RNA aptamer selection. In this study three different reverse transcriptases were investigated towards the enzymatic recognition of LNA nucleotides. Both incorporation as well as reading capabilities of the LNA nucleotides was investigated to fully understand the limitations of the enzymatic recognition. We found that SuperScript® III Reverse Transcriptase is an efficient enzyme for the recognition of LNA nucleotides, making it a prime candidate to be used in de novo selection of LNA containing RNA aptamers.

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Jan 1, 1994·Molecular Biology Reports·S J Klug, M Famulok
Feb 8, 2003·Trends in Biotechnology·Michael Petersen, Jesper Wengel
Jan 22, 2005·Annual Review of Medicine·Shahid M NimjeeBruce A Sullenger
Mar 7, 2006·Nature Reviews. Drug Discovery·Eugene W M NgAnthony P Adamis
Oct 31, 2006·Analytical and Bioanalytical Chemistry·Subash Chandra Bose Gopinath
Feb 23, 2007·Chembiochem : a European Journal of Chemical Biology·Rakesh N VeeduJesper Wengel
Jul 14, 2007·Biomolecular Engineering·Regina StoltenburgBeate Strehlitz
Aug 25, 2007·Chemical Reviews·Michael FamulokGünter Mayer
Jun 7, 2008·Journal of the American Chemical Society·Rakesh N VeeduJesper Wengel
Mar 21, 2009·Organic & Biomolecular Chemistry·Rakesh N VeeduJesper Wengel
May 22, 2009·RNA Biology·Rakesh N Veedu, Jesper Wengel
Mar 17, 2010·Chemistry & Biodiversity·Rakesh N Veedu, Jesper Wengel
Oct 12, 2010·Bioorganic & Medicinal Chemistry Letters·Rakesh N VeeduJesper Wengel

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Citations

Sep 22, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Stella Diafa, Marcel Hollenstein
May 15, 2015·Chemical Communications : Chem Comm·Stacey L EdwardsRakesh N Veedu
Sep 18, 2013·Artificial DNA, PNA & XNA·Masayasu Kuwahara, Satoshi Obika
Mar 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Bharat N GawandeNebojsa Janjic
Nov 28, 2012·Nucleic Acid Therapeutics·Kasper K Karlsen, Jesper Wengel
Feb 13, 2018·RNA Biology·Jyoti BalaHoshang Unwalla
Dec 12, 2017·ChemistrySelect·George MaioPrabodhika Mallikaratchy
Jul 28, 2020·Quarterly Reviews of Biophysics·Ali NikoomanzarJohn C Chaput
Jun 7, 2019·Nucleic Acids Research·Lynnette N JacksonJohn C Chaput
Jul 13, 2020·Trends in Biotechnology·Samara Martín-AlonsoLuis Menéndez-Arias
Oct 27, 2021·Nucleosides, Nucleotides & Nucleic Acids·Muthian ShanmugasundaramAnilkumar R Kore

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Methods Mentioned

BETA
SELEX
PCR
electrophoresis

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