Efficient rhizosphere colonization by Pseudomonas fluorescens f113 mutants unable to form biofilms on abiotic surfaces

Environmental Microbiology
Emma BarahonaRafael Rivilla

Abstract

Motility is a key trait for rhizosphere colonization by Pseudomonas fluorescens. Mutants with reduced motility are poor competitors, and hypermotile, more competitive phenotypic variants are selected in the rhizosphere. Flagellar motility is a feature associated to planktonic, free-living single cells, and although it is necessary for the initial steps of biofilm formation, bacteria in biofilm lack flagella. To test the correlation between biofilm formation and rhizosphere colonization, we have used P. fluorescens F113 hypermotile derivatives and mutants affected in regulatory genes which in other bacteria modulate biofilm development, namely gacS (G), sadB (S) and wspR (W). Mutants affected in these three genes and a hypermotile variant (V35) isolated from the rhizosphere were impaired in biofilm formation on abiotic surfaces, but colonized the alfalfa root apex as efficiently as the wild-type strain, indicating that biofilm formation on abiotic surfaces and rhizosphere colonization follow different regulatory pathways in P. fluorescens. Furthermore, a triple mutant gacSsadBwspR (GSW) and V35 were more competitive than the wild-type strain for root-tip colonization, suggesting that motility is more relevant in this environment...Continue Reading

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Citations

Jul 3, 2013·Applied and Environmental Microbiology·Sarah C SeatonStuart B Levy
Jan 17, 2012·Applied and Environmental Microbiology·David LalaounaWafa Achouak
Jan 3, 2012·Applied and Environmental Microbiology·Ana M Abarca-GrauRamón Penyalver
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