Efficient serum-free derivation of oligodendrocyte precursors from neural stem cell-enriched cultures.

Stem Cells
Rajesh C RaoRonald D McKay

Abstract

Oligodendrocytes derived in the laboratory from stem cells have been proposed as a treatment for acute and chronic injury to the central nervous system. Platelet-derived growth factor (PDGF) receptor alpha (PDGFRalpha) signaling is known to regulate oligodendrocyte precursor cell numbers both during development and adulthood. Here, we analyze the effects of PDGFRalpha signaling on central nervous system (CNS) stem cell-enriched cultures. We find that AC133 selection for CNS progenitors acutely isolated from the fetal cortex enriches for PDGF-AA-responsive cells. PDGF-AA treatment of fibroblast growth factor 2-expanded CNS stem cell-enriched cultures increases nestin(+) cell number, viability, proliferation, and glycolytic rate. We show that a brief exposure to PDGF-AA rapidly and efficiently permits the derivation of O4(+) oligodendrocyte-lineage cells from CNS stem cell-enriched cultures. The derivation of oligodendrocyte-lineage cells demonstrated here may support the effective use of stem cells in understanding fate choice mechanisms and the development of new therapies targeting this cell type.

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Citations

Apr 30, 2013·Stem Cells and Development·Walaa F AlsanieSteven Petratos
Sep 1, 2015·Journal of Neurochemistry·Osamu ImamuraKunio Takishima
Aug 19, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Mehnaz KhanRajesh C Rao
Jul 4, 2012·Cancer Science·Kazuya MotomuraToshihiko Wakabayashi
Oct 7, 2014·The FEBS Journal·Anqi XiongKarin Forsberg-Nilsson
Oct 17, 2009·Brain Pathology·Filippo Calzolari, Paolo Malatesta
Jun 17, 2015·Brain Tumor Pathology·Melissa RanjitAtsushi Natsume
Jun 23, 2018·Acta Biochimica Et Biophysica Sinica·Wenguang Chang, Junfang Teng

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