Efficient, Traceless Semi-Synthesis of α-Synuclein Labeled with a Fluorophore/Thioamide FRET Pair

Synlett : Accounts and Rapid Communications in Synthetic Organic Chemistry
Rebecca F WissnerE James Petersson

Abstract

We have shown that thioamides can be incorporated into proteins through semi-synthesis and used as probes to monitor structural changes. To date, our methods have required the presence of a cysteine at the peptide ligation site, which may not be present in the native peptide sequence. Here, we present a strategy for the semi-synthesis of thioproteins using homocysteine as a ligation point with subsequent masking as methionine, making the ligation "traceless."

Citations

Jan 11, 2018·Biophysical Journal·John J FerrieE James Petersson
Nov 26, 2013·Journal of the American Chemical Society·Jacob M GoldbergE James Petersson
Aug 15, 2014·ACS Chemical Biology·Jitesh A Soares, Laura L Kiessling
Jan 31, 2019·ACS Chemical Biology·Nilkamal MahantaDouglas A Mitchell

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