Efficient transduction of primary vascular cells by the rare adenovirus serotype 49 vector

Human Gene Therapy
Rachel S DakinAndrew H Baker

Abstract

Neointima formation and vascular remodeling through vascular smooth muscle cell migration and proliferation can limit the long-term success of coronary interventions, for example, in coronary artery bypass grafting (CABG). Ex vivo gene therapy has the potential to reduce unnecessary cell proliferation and limit neointima formation in vascular pathologies. To date, the species C adenovirus serotype 5 has been commonly used for preclinical gene therapy; however, its suitability is potentially limited by relatively poor tropism for vascular cells and high levels of preexisting immunity in the population. To avoid these limitations, novel species of adenovirus are being tested; here we investigate the potential of adenovirus 49 (Ad49) for use in gene therapy. Transduction of primary human vascular cells by a range of adenovirus serotypes was assessed; Ad49 demonstrated highest transduction of both vascular smooth muscle and endothelial cells. Gene transfer with Ad49 in vascular smooth muscle and endothelial cells was possible following short exposure times (<1 hr) and with low MOI, which is clinically relevant. Ex vivo delivery to surplus CABG tissue showed efficient gene transfer with Ad49, consistent with the in vitro findings. L...Continue Reading

References

Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·H E von der LeyenV J Dzau
Sep 1, 1995·Circulation Research·A H SchulickD A Dichek
Jul 2, 1998·Circulation Research·M D RekhterD Gordon
Sep 8, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·S A NicklinA H Baker
Oct 21, 2003·Nature Medicine·Anuj GaggarAndré Lieber
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shawn M SumidaDan H Barouch
Sep 8, 2005·Journal of Clinical Microbiology·Ulrike BauerWerner Seidel
Jul 4, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Virendra I PatelChristiane Ferran
Oct 6, 2006·Journal of Clinical Microbiology·Anna R ThornerDan H Barouch
Sep 11, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Angelika B KritzAndrew H Baker
Oct 24, 2008·Journal of Virology·Alan L ParkerAndrew H Baker
Mar 31, 2009·Journal of Cardiac Failure·Brian E JaskiUNKNOWN Calcium Up-Regulation by Percutaneous Administration of Gene Therapy In Cardiac Disease (CUPID) Trial Investigators
Oct 10, 2009·Circulation Research·Rajesh GuptaDouglas W Losordo
Jun 29, 2011·Circulation·Mariell JessupUNKNOWN Calcium Upregulation by Percutaneous Administration of Gene Therapy in Cardiac Disease (CUPID) Investigators
Jul 28, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christina A Pacak, Barry J Byrne
Jun 1, 2012·Methods in Molecular Biology·Raul AlbaStuart A Nicklin
Aug 14, 2013·Journal of Cardiothoracic Surgery·Katie M WhiteAndrew H Baker
Dec 12, 2013·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Subodh VermaStephen E Fremes

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Citations

Oct 20, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Julio Alonso-PadillaAndrew H Baker
May 23, 2019·Human Genetics·Minyoung Lee, Hyongbum Kim
Sep 14, 2019·Science Advances·Alexander T BakerAlan L Parker
Feb 25, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dayananda KasalaChae-Ok Yun
May 30, 2021·The Journal of Gene Medicine·Wenfeng ZhangZhuozhuang Lu

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Methods Mentioned

BETA
coronary artery bypass
FCS
confocal microscopy

Software Mentioned

FACS DIVA
CUPID

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