Efficient transfer of human adipose-derived stem cells by chitosan/gelatin blend films

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Nai-Chen ChengTai-Horng Young

Abstract

Adipose-derived stem cells (ASCs) are a potential source of abundant mesenchymal stem cells and represent a promising cell-based therapy for tissue damage or degeneration conditions. Previous investigations have demonstrated enhanced therapeutic effects of ASCs in a three-dimensional spheroid culture formulation. In this study, we hypothesize that a composite membrane made of chitosan/gelatin (C/G) is beneficial to facilitate transfer of human ASCs in spheroids. Increasing chitosan content within the blends enhanced the mechanical properties of the sample, including tensile strength and elongation-at-break ratio. Although ASC spheroids developed shortly after seeding on pure chitosan films, increasing gelatin proportion in the C/G blends promoted cell adhesion onto the membranes. We also found that ASCs did not proliferate on chitosan films, but C/G blends of different ratios supported ASC proliferation in the first 4 days of culture. However, ASCs on all C/G blends started to detach from the films to form spheroids after day 4, while ASCs on pure gelatin films remained attached and continued to grow. Gradual gelatin release from the C/G blend films, leading to enriched chitosan content in the blends, probably encouraged ASC de...Continue Reading

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Citations

Jul 15, 2015·Biotechnology Letters·Kai Su, Chunming Wang
Mar 30, 2020·Tissue Engineering. Part B, Reviews·Greet MerckxAnnelies Bronckaers
May 13, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sarah J FitzgeraldAmol V Janorkar
May 5, 2020·Journal of Biomedical Materials Research. Part a·Yang LiuGuangwei Sun
Dec 6, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Manuela CabiatiSilvia Del Ry
Sep 18, 2017·Carbohydrate Polymers·Ehsan ZeimaranNahrizul Adib Kadri

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