PMID: 11328598May 1, 2001Paper

Efficient transfer of intact oligonucleotides into the nucleus of ligament scar fibroblasts by HVJ-cationic liposomes is correlated with effective antisense gene inhibition

Journal of Biochemistry
N NakamuraYasufumi Kaneda

Abstract

The efficacy of two different cationic liposomes, Lipofectin and hemagglutinating virus of Japan (HVJ)-cationic liposomes, on nuclear uptake of fluorescence-labeled phosphorothioate oligodeoxyribonucleotide (S-ODN) by ligament scar fibroblasts and suppression of decorin mRNA expression when antisense decorin S-ODN was transferred was investigated. There was no significant difference in nuclear uptake of fluorescent ODN between the two methods. However, only HVJ-cationic liposomes had a significant effect on suppression of decorin mRNA expression levels. To address the discrepancy, the molecular integrity of the transferred ODN in the cells was assessed by analysis of fluorescence resonance energy transfer (FRET) within double-fluorescence-labeled S-ODN. More than 70% of the ODN transfected by HVJ-cationic liposomes remained intact within the nucleus at 20 h after transfection, while the majority of the ODN transferred by Lipofectin was degraded at this point. These results suggest a strong relationship between the nuclear integrity of transfected antisense ODN and its suppression of target mRNA expression.

Citations

Dec 10, 2009·Pharmaceutical Research·Paul E LundChava Kimchi-Sarfaty
Nov 5, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Lauren B SloaneGerald E McClearn
Mar 30, 2011·Advanced Drug Delivery Reviews·Yasufumi Kaneda
Dec 7, 2006·The Journal of Gene Medicine·Saroj SuvanasuthiYasufumi Kaneda
Sep 27, 2003·Tissue Engineering·James Cho-Hong GohEng-Hin Lee
Apr 25, 2006·Cancer Science·Yasufumi Kaneda, Yasuhiko Tabata
Dec 14, 2001·The Journal of Biological Chemistry·Venkat GopalanSidney Altman
Jul 16, 2003·Scandinavian Journal of Medicine & Science in Sports·S P MagnussonM Kjaer
Oct 5, 2011·Cold Spring Harbor Protocols·Yasufumi Kaneda

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