PMID: 2123464Oct 1, 1990Paper

Efficient transformation of chicken embryo fibroblasts by c-Jun requires structural modification in coding and noncoding sequences

Genes & Development
T J BosP K Vogt

Abstract

To assess the transforming capability of the c-Jun protein, we introduced the chicken c-jun proto-oncogene into a replication competent avian retroviral expression vector (RCAS). Viral Jun efficiently transformed chicken embryo fibroblasts (CEFs) when expressed from this vector. Overexpression of c-Jun leads to transformation of CEFs with an efficiency that is 15- to 25-fold less than that seen for v-Jun, suggesting that v-Jun contains structural features that increase its oncogenic potential relative to c-Jun. There are four structural differences between v-Jun and c-Jun. To determine the relative contribution that each of these structural differences between v-Jun and c-Jun has on oncogenic activity, several deletion and substitution mutants were constructed. Each of these mutants was expressed in CEF and assayed for transformation by focus formation. Analysis of the results reveals that deletion of a region of 27 amino acids near the amino terminus of c-Jun and deletion of 3'-untranslated sequences are critical in activating the full oncogenic potential of Jun.

References

Jan 1, 1990·Advances in Cancer Research·P K Vogt, T J Bos
Aug 18, 1988·Nature·W W LamphI M Verma
Dec 1, 1988·Genes & Development·F J RauscherT Curran
May 1, 1989·Trends in Biochemical Sciences·P K Vogt, T J Bos
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J SchütteM J Birrer
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·A R BallP K Vogt
Dec 15, 1988·Nature·T Kouzarides, E Ziff
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y MakiP K Vogt
May 20, 1988·Science·F J RauscherB R Franza
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K Ryder, D Nathans
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K RyderD Nathans
Mar 1, 1974·Journal of Clinical Pathology·G V Heimer, C E Taylor
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein

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Citations

Sep 27, 2000·International Journal of Cancer. Journal International Du Cancer·J Rinehart-KimT Bos
Jan 29, 2002·Analytical Biochemistry·René KizekFrantisek Jelen
Dec 10, 1991·Biochimica Et Biophysica Acta·P Angel, M Karin
Aug 8, 1996·Biochimica Et Biophysica Acta·A IsakssonD Bohmann
Jun 1, 1991·Current Opinion in Cell Biology·J Ghysdael, M Yaniv
Oct 2, 2001·Pharmacology & Therapeutics·M LeszczynieckaP B Fisher
Aug 5, 1998·Pharmacology & Therapeutics·M Scheffner
Apr 1, 1996·Immunology and Cell Biology·V C Foletta
Aug 22, 2002·Nature Reviews. Cancer·Peter K Vogt
Jun 4, 2008·Oncogene·H Jiang, P K Vogt
Nov 14, 2007·Oncogene·A DenleyP K Vogt
Aug 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Minghao SunPeter K Vogt
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jonathan R HartPeter K Vogt
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M CastellazziG Brun
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Chida, P K Vogt
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A SchönthalW Eckhart
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·V AdlerA S Kraft
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L S HåvarsteinP K Vogt
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Li, P K Vogt
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T KamedaH Iba
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·M Hartl, K Bister
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·S ScheidlerP K Vogt
Apr 1, 1997·The EMBO Journal·E Bossy-WetzelM Yaniv
Jun 8, 2001·Molecular and Cellular Biology·S LeppäD Bohmann
Oct 1, 2009·Physiological Reviews·Sven Loebrich, Elly Nedivi
Oct 12, 2000·Biochemical and Biophysical Research Communications·Y XiaJ Li
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sohye KangPeter K Vogt
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·J LiP K Vogt
Mar 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Marco GymnopoulosPeter K Vogt
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·H WatanabeH Iba
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·M HartlK Bister

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