Efficient transformation of Kalanchoe blossfeldiana and production of male-sterile plants by engineered anther ablation.

Plant Cell Reports
Begoña García-SogoLuis A Cañas

Abstract

Engineered male sterility in ornamental plants has many applications such as facilitate hybrid seed production, eliminate pollen allergens, reduce the need for deadheading to extend the flowering period, redirect resources from seeds to vegetative growth, increase flower longevity and prevent gene flow between genetically modified and related native plants. We have developed a reliable and efficient Agrobacterium-mediated protocol for the genetic transformation of different Kalanchoe blossfeldiana commercial cultivars. Transformation efficiency for cv. 'Hillary' was 55.3% whereas that of cv. 'Tenorio' reached 75.8%. Selection was carried out with the nptII gene and increasing the kanamycin concentration from 25 to 100 mg l(-1) allowed to reduced escapes from 50 to 60% to virtually 0%. This method was used to produce male-sterile plants through engineered anther ablation. In our approach, we tested a male sterility chimaeric gene construct (PsEND1::barnase) to evaluate its effectiveness and effect on phenotype. No significant differences were found in the growth patterns between the transgenic lines and the wild-type plants. No viable pollen grains were observed in the ablated anthers of any of the lines carrying the PsEND1::bar...Continue Reading

References

Mar 1, 1996·Current Biology : CB·W ChiuJ Sheen
Oct 27, 1997·The Plant Cell·T P Beals, R B Goldberg
Dec 17, 2008·Journal of Plant Physiology·Nicole GardnerAlan G Smith

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Citations

Sep 1, 2012·BMC Plant Biology·Begoña García-SogoLuis Antonio Cañas
Jul 11, 2013·Plant Reproduction·María Dolores GómezLuis A Cañas
Mar 11, 2011·Trends in Biotechnology·Charles KwitC Neal Stewart
Apr 16, 2013·Plant Biotechnology Journal·Mónica MedinaConcepción Gómez-Mena
Oct 28, 2015·Plant Biotechnology Journal·Reginald J MillwoodCharles Neal Stewart
Feb 25, 2014·Trends in Plant Science·Anne M BorlandJohn C Cushman
Oct 30, 2015·Malaria Journal·Erum DilshadBushra Mirza
Feb 14, 2020·Plant Cell Reports·M Valentina BrionesMatthias Fladung
Jul 12, 2019·Frontiers in Plant Science·Edelín RoqueLuis A Cañas
Apr 16, 2016·Current Opinion in Plant Biology·James HartwellSusanna F Boxall

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