Efficient transformation of pseudomonas strains with pNI vectors by electroporation

Bioscience, Biotechnology, and Biochemistry
N ItohY Koide

Abstract

The optimum conditions for electro-transformation of some Pseudomonas strains including P. putida, P. fluorescens, and P. flavida, were defined using the pNI105 vector, resulting in 100-10,000 fold increases in transformation efficiency compared with conventional chemical transformation with MgCl2. The growth phase of the cultured cells and the field strength were important in obtaining high transformation efficiency. Under optimal conditions, 3.2 x 10(7) transformants per 1 microgram DNA were obtained using a combination of pNI105 and P. fluorescens IAM12022. This value is the highest ever obtained for a Pseudomonas species. In addition, the procedures were applicable to the effective transformation of fluorescent Pseudomonas strains with pNI vectors.

References

Dec 1, 2009·Applied Microbiology and Biotechnology·Trond Erik Vee Aune, Finn Lillelund Aachmann
Oct 5, 2011·Protein Expression and Purification·Diane M RetallackLawrence Chew
Mar 10, 2016·Antimicrobial Agents and Chemotherapy·Renata GalettiAna Lúcia Costa Darini
Feb 15, 2015·Applied and Environmental Microbiology·Chiho Suzuki-MinakuchiHideaki Nojiri
Dec 17, 2002·The Journal of General and Applied Microbiology·Fumiyasu Fukumori, Mitsuru Kishii

Related Concepts

Pseudomonas Infections
Transformation, Genetic
Shuttle Vectors
Pseudomonas putida
Fluorescent stain
Platylomia flavida
Magnesium Chloride
Electroporation
Pseudomonas species
Psychomyia flavida

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