Efficient transformation of the beetle Tribolium castaneum using the Minos transposable element: quantitative and qualitative analysis of genomic integration events

Genetics
Anastasios PavlopoulosMartin Klingler

Abstract

Genetic transformation in insects holds great promise as a tool for genetic manipulation in species of particular scientific, economic, or medical interest. A number of transposable elements have been tested recently as potential vectors for transformation in a range of insects. Minos is one of the most promising elements because it appears to be active in diverse species and has the capacity to carry large inserts. We report here the use of the Minos element as a transformation vector in the red flour beetle Tribolium castaneum (Coleoptera), an important species for comparative developmental and pest management studies. Transgenic G(1) beetles were recovered from 32.4% of fertile G(0)'s injected with a plasmid carrying a 3xP3-EGFP-marked transposon and in vitro synthesized mRNA encoding the Minos transposase. This transformation efficiency is 2.8-fold higher than that observed when using a plasmid helper. Molecular and genetic analyses show that several independent insertions can be recovered from a single injected parent, but that the majority of transformed individuals carry single Minos insertions. These results establish Minos as one of the most efficient vectors for genetic transformation in insects. In combination with p...Continue Reading

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C J O'Kane, W J Gehring
Oct 22, 1982·Science·G M Rubin, A C Spradling
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·A C SpradlingG M Rubin
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·T G LoukerisC Savakis
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·G FranzC Savakis
Jan 1, 1993·Archives of Insect Biochemistry and Physiology·A M HandlerD A O'Brochta
Jul 14, 1998·Insect Molecular Biology·M AshburnerJ J Peloquin
Oct 6, 1998·Annual Review of Biochemistry·R Y Tsien
Jun 17, 1999·Development Genes and Evolution·A BerghammerM Klingler
Aug 4, 1999·Trends in Genetics : TIG·R H PlasterkZ Ivics
Dec 10, 1999·Nature·A J BerghammerE A Wimmer
Dec 9, 2000·Annual Review of Entomology·P W AtkinsonD A O'Brochta
Jan 11, 2001·Development Genes and Evolution·C HornE A Wimmer
Jan 11, 2001·Development Genes and Evolution·C Horn, E A Wimmer
Feb 13, 2001·Insect Biochemistry and Molecular Biology·A M Handler
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·L ZagoraiouC Savakis
Apr 25, 2002·Nature Reviews. Genetics·Melissa D Adams, Jeff J Sekelsky
May 25, 2002·Nature·Junitsu ItoMarcelo Jacobs-Lorena
Jul 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·François HuetWilliam M Gelbart
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Carsten HornErnst A Wimmer
Oct 24, 2002·Development, Growth & Differentiation·Hongjie ZhangSumihare Noji
Dec 17, 2002·Nature Biotechnology·Masahiro TomitaKatsutoshi Yoshizato
Mar 7, 2003·Genomics·Dubravka DrabekFrank Grosveld
Mar 26, 2003·Insect Biochemistry and Molecular Biology·M SumitaniM Hatakeyama
Jun 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yasunori SasakuraNori Satoh
Jun 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Udo HackerErnst A Wimmer
Sep 17, 2003·Insect Molecular Biology·M D LorenzenR W Beeman

❮ Previous
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Citations

Aug 15, 2012·Development Genes and Evolution·Johannes Benno SchinkoGregor Bucher
Jan 12, 2007·Molecular Genetics and Genomics : MGG·K UchinoT Tamura
Nov 15, 2006·Transgenic Research·Maria MarkakiCharalambos Savakis
Mar 30, 2010·Médecine sciences : M/S·François Bonneton
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anastasios PavlopoulosMichalis Averof
Jan 20, 2011·Genes & Development·Jeremy A Lynch, Siegfried Roth
May 21, 2010·BMC Developmental Biology·Johannes B SchinkoGregor Bucher
Nov 18, 2010·BMC Molecular Biology·Ian A WarrenHazel Smith
Jun 24, 2005·Genetics·Athanasios MetaxakisCharalambos Savakis
May 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Anastasios Pavlopoulos, Michalis Averof
Dec 14, 2011·Annual Review of Entomology·Malcolm J Fraser
Jan 11, 2014·Biochemistry·Maryia TrubitsynaJulia M Richardson
Jul 16, 2008·Insect Biochemistry and Molecular Biology·Kendra S SiebertRichard W Beeman
Nov 6, 2007·Developmental and Comparative Immunology·Boran AltincicekAndreas Vilcinskas
Nov 21, 2007·Molecular Phylogenetics and Evolution·David R Angelini, Elizabeth L Jockusch
Apr 19, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Wim G M Damen
Feb 24, 2007·Insect Molecular Biology·M D LorenzenR W Beeman
May 15, 2013·Insect Molecular Biology·C SchulteM Beye
Dec 17, 2009·Molecular Ecology·Christopher D SmithJürgen Gadau
Oct 6, 2005·Trends in Neurosciences·Scott J DouglasMarla B Sokolowski
May 30, 2015·Fungal Genetics and Biology : FG & B·Minoas EvangelinosGeorge Diallinas
Mar 18, 2008·Insect Biochemistry and Molecular Biology·Patamarerk EngsontiaStephen Brown
Mar 18, 2008·Insect Biochemistry and Molecular Biology·Jianjun WangYoonseong Park
Jan 24, 2014·Bioarchitecture·Matthew A Benton, Anastasios Pavlopoulos
Oct 26, 2016·ELife·Frederike AlwesMichalis Averof
Sep 22, 2017·Frontiers in Physiology·Dan SunYoujun Zhang
May 14, 2020·Current Pharmaceutical Design·Janin RösnerHans Merzendorfer
May 26, 2011·Development·Zacharias KontarakisMichalis Averof
Feb 12, 2010·Cold Spring Harbor Protocols·Susan J BrownMartin Klingler

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