Mar 7, 1983

Efficient translation and polyribosome binding of 125I-labelled rabbit globin messenger ribonucleoprotein

FEBS Letters
P D Butcher, H R Arnstein

Abstract

Rabbit polyribosomal globin messenger ribonucleoprotein (mRNP) was labelled under mild conditions, using 125I and Iodogen, in the protein moiety so that the fate of mRNA-associated proteins could be followed during translation. 125I-mRNP was shown to retain functional activity in the nuclease-treated reticulocyte lysate translation system under optimal labelling conditions. Polyribsome binding of 125I-mRNP and its sensitivity to cycloheximide indicated a functional- and translation-dependent binding of mRNP proteins. The results constitute a successful and direct approach to the study of mRNA-associated proteins in translational control.

Mentioned in this Paper

Polyribosomes
Nucleoproteins
Isotope Labeling, Stable
Protein Biosynthesis
Pedameth
Reticulocytes
Globin
Iodine Radioisotopes
Poly(A) Tail
Actidione

About this Paper

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