Efficiently editing the vaccinia virus genome by using the CRISPR-Cas9 system

Journal of Virology
Ming YuanYaohe Wang

Abstract

Vaccinia virus (VACV) continues to be used in immunotherapy for the prevention of infectious diseases and treatment of cancer since its use for the eradication of smallpox. However, the current method of editing the VACV genome is not efficient. Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs (gRNAs) targeting all VACV genes will be valuable for the study of VACV gene functions.

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Citations

Sep 30, 2015·Molecular Therapy. Methods & Clinical Development·Ming YuanYaohe Wang
Oct 25, 2016·Biochimica Et Biophysica Acta·Lang Yi, Jinming Li
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