Efflux-mediated resistance to arsenicals in arsenic-resistant and -hypersensitive Chinese hamster cells

Toxicology and Applied Pharmacology
Z WangT G Rossman

Abstract

Several Chinese hamster V79 cell line variants resistant to arsenite and one arsenite-hypersensitive variant have been isolated. The basis for the variation in arsenite sensitivity was studied by transport experiments using radiolabeled arsenite. Two arsenite-resistant variants (As/R7 and As/R27) exhibited decreased accumulation of arsenite, and the hypersensitive variant (As/S5) exhibited increased arsenite accumulation compared with the parental line. Cells depleted of endogenous energy reserves were loaded with radiolabeled arsenite, and the rate of arsenic efflux was measured. Arsenite-resistant variants exhibited an increased rate of efflux, while the hypersensitive variant exhibited a decreased efflux rate. Efflux was decreased in cells incubated with the protonophore carbonyl cyanide m-chlorophenylhydrazine, demonstrating its energy dependence. Two inhibitors of glutathione S-transferase also decreased arsenite efflux, suggesting the involvement of an arsenite-glutathione complex. However, separation of the products of extrusion and the intracellular arsenic species by paper chromatography followed by autoradiography failed to show the appearance of an arsenite-glutathione complex in either case. Rather, all label in the...Continue Reading

Citations

Nov 5, 1998·The New England Journal of Medicine·S L SoignetR P Warrell
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·R MukhopadhyayB P Rosen
Dec 12, 2003·Journal of Toxicology and Environmental Health. Part a·P Robinan GentryHarvey J Clewell
Apr 1, 2005·Journal of Toxicology and Environmental Health. Part a·P Robinan GentryHarvey J Clewell
Jan 1, 1997·Annual Review of Pharmacology and Toxicology·H V Aposhian
Oct 20, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rui WangYongkui Jing
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M GhoshB P Rosen
Dec 15, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Yong LongZongbin Cui
Dec 23, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Zhi-Feng MiaoTsui-Chun Tsou
Aug 19, 2007·The New Phytologist·X Y XuF J Zhao
Feb 4, 1998·Chemico-biological Interactions·T Gebel
Nov 20, 2016·Protein Science : a Publication of the Protein Society·Lorien J ParkerMichael W Parker
Dec 31, 1997·The Journal of Biological Chemistry·R WysockiS Ułaszewski
Jan 7, 1999·Biological Trace Element Research·N DolzhanskayaT G Rossman
Aug 26, 1998·The Journal of Biological Chemistry·B Kurdi-HaidarS B Howell
May 30, 2020·Environmental Toxicology and Pharmacology·Atish RayShibnath Mazumder
Jun 1, 2000·Journal of Inorganic Biochemistry·T L ZhangK Wang
Jun 4, 1999·Trends in Microbiology·B P Rosen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.