PMID: 9642183Jun 27, 1998Paper

Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-T1E

Journal of Bacteriology
J L RamosA Segura

Abstract

The basic mechanisms underlying solvent tolerance in Pseudomonas putida DOT-T1E are efflux pumps that remove the solvent from bacterial cell membranes. The solvent-tolerant P. putida DOT-T1E grows in the presence of high concentrations (e.g., 1% [vol/vol]) of toluene and octanol. Growth of P. putida DOT-T1E cells in LB in the presence of toluene supplied via the gas phase has a clear effect on cell survival: the sudden addition of 0.3% (vol/vol) toluene to P. putida DOT-T1E pregrown with toluene in the gas phase resulted in survival of almost 100% of the initial cell number, whereas only 0.01% of cells pregrown in the absence of toluene tolerated exposure to this aromatic hydrocarbon. One class of toluene-sensitive octanol-tolerant mutant was isolated after Tn5-'phoA mutagenesis of wild-type P. putida DOT-T1E cells. The mutant, called P. putida DOT-T1E-18, was extremely sensitive to 0.3% (vol/vol) toluene added when cells were pregrown in the absence of toluene, whereas pregrowth on toluene supplied via the gas phase resulted in survival of about 0.0001% of the initial number. Solvent exclusion was tested with 1,2,4-[14C]trichlorobenzene. The levels of radiochemical accumulated in wild-type cells grown in the absence and in the...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·O Lomovskaya, K Lewis
Aug 15, 1991·Biochemical and Biophysical Research Communications·Y LeiT Nakae
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A A NeyfakhL B Chen
Nov 1, 1989·Antimicrobial Agents and Chemotherapy·H Nikaido
Aug 1, 1988·Antimicrobial Agents and Chemotherapy·S P CohenS B Levy
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·F C FranklinK N Timmis
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L McMurryS B Levy
Jun 1, 1995·Microbiological Reviews·J SikkemaB Poolman
Oct 1, 1994·The Journal of Antimicrobial Chemotherapy·K Poole
Dec 1, 1994·Trends in Microbiology·D MaH Nikaido
Oct 1, 1994·FEMS Microbiology Letters·N GotohT Nishino
Oct 1, 1996·Journal of Bacteriology·H Nikaido
Oct 1, 1996·Journal of Bacteriology·S Isken, J A de Bont
Feb 14, 1997·The Journal of Biological Chemistry·J L RamosA Fernández-Barrero
Sep 18, 1997·Applied and Environmental Microbiology·R Aono, H Kobayashi
Jan 22, 1998·Applied and Environmental Microbiology·M J HuertasJ L Ramos
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

❮ Previous
Next ❯

Citations

Dec 30, 1998·FEMS Microbiology Letters·H HirayamaK Horikoshi
Dec 1, 2000·Applied and Environmental Microbiology·T BuggM R Gray
Aug 30, 2001·Applied and Environmental Microbiology·M I Ramos-GonzálezJ L Ramos
Jul 27, 2002·Annual Review of Microbiology·Juan L RamosAna Segura
Nov 29, 2002·Microbiology and Molecular Biology Reviews : MMBR·Steve GrkovicRonald A Skurray
Mar 17, 2004·FEMS Microbiology Letters·Florence JudeClaudine Quentin
Oct 21, 2005·Extremophiles : Life Under Extreme Conditions·Zileena ZahirJonathan J Dennis
Oct 31, 2006·Journal of Bacteriology·Estrella DuqueJuan-Luis Ramos
Nov 8, 2007·Molecular Microbiology·Wilson TeránMaría-Trinidad Gallegos
Jun 1, 2010·Environmental Microbiology Reports·Vanina GarcíaAna Segura
May 3, 2015·FEMS Microbiology Reviews·Juan-Luis RamosAmalia Roca
Jul 16, 2016·Microbiology·Carlos Molina-SantiagoEstrella Duque
May 16, 2018·Biotechnology for Biofuels·Georg BaslerJay Keasling
Oct 29, 2000·Journal of Bacteriology·H KobayashiK Horikoshi
Nov 30, 2000·The Journal of Biological Chemistry·J WeryJ A de Bont
Dec 20, 2002·Biotechnology and Bioengineering·A AmanullahJ M Woodley
Sep 4, 2004·Environmental Microbiology·Estrella DuqueJuan-Luis Ramos
Jan 13, 2004·Molecular Plant-microbe Interactions : MPMI·Antje BurseMatthias S Ullrich
Jan 13, 2006·The Journal of Biological Chemistry·Wilson TeránMaría-Trinidad Gallegos
Mar 11, 2004·Environmental Microbiology·Ana SeguraJuan-Luis Ramos
Jan 22, 2003·Environmental Microbiology·José Ignacio JiménezEduardo Díaz
Oct 16, 2002·Biotechnology and Bioengineering·In Seon KimMurray R Gray

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.