Abstract
Quaternary ammonium compounds (QACs) are commonly used antiseptics that are now known to be subject to bacterial resistance. The prevalence and mechanisms of such resistance, however, remain underexplored. We investigated a variety of QACs, including those with multicationic structures (multiQACs), and the resistance displayed by a variety of Staphylococcus aureus strains with and without genes encoding efflux pumps, the purported main driver of bacterial resistance in MRSA. Through minimum inhibitory concentration (MIC)-, kinetic-, and efflux-based assays, we found that neither the qacR/qacA system present in S. aureus nor another efflux pump system is the main reason for bacterial resistance to QACs. Our findings suggest that membrane composition could be the predominant driver that allows CA-MRSA to withstand the assault of conventional QAC antiseptics.
References
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·I T PaulsenR A Skurray
Nov 18, 1998·The Journal of Antimicrobial Chemotherapy·J A GrandoniJ V Schloss
Dec 12, 2001·Science·M A SchumacherR G Brennan
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·P D FeyP M Schlievert
Dec 29, 2004·International Journal of Antimicrobial Agents·Maria Braoudaki, Anthony C Hilton
May 3, 2005·Research in Microbiology·Derie E FuentesClaudio C Vásquez
Sep 16, 2005·Journal of Applied Microbiology·P Gilbert, L E Moore
Jan 5, 2006·Journal of Clinical Microbiology·Fred C TenoverPaul M Dunman
Jun 28, 2006·Antimicrobial Agents and Chemotherapy·A S BayerM R Yeaman
Jul 11, 2006·Journal of Clinical Microbiology·Hsin HuangStuart H Cohen
Oct 25, 2006·Antimicrobial Agents and Chemotherapy·Christopher J IoannouStephen P Denyer
Jan 30, 2008·The Journal of Antimicrobial Chemotherapy·Leila ValiSebastian G B Amyes
Dec 23, 2008·International Journal of Antimicrobial Agents·Emmanuel Frempong-MansoGlenn W Kaatz
Mar 16, 2011·Antimicrobial Agents and Chemotherapy·Jean LongtinRoberto G Melano
Oct 29, 2011·BMC Microbiology·Sofia Santos CostaIsabel Couto
Feb 4, 2012·The Journal of Infectious Diseases·Justine K RudkinRuth C Massey
Mar 17, 2012·International Journal of Antimicrobial Agents·Sylvie Buffet-BataillonAnne Jolivet-Gougeon
Feb 15, 2013·Current Protocols in Microbiology·Nicholas P Vitko, Anthony R Richardson
May 15, 2013·Antimicrobial Agents and Chemotherapy·Leonardo FuriMarco Rinaldo Oggioni
Feb 11, 2014·Biomacromolecules·Federica SgolastraGregory N Tew
Sep 1, 2014·International Journal of Food Microbiology·Dongyang XuHe Yan
May 20, 2015·ChemMedChem·Yasmeen AbouelhassanRobert W Huigens
Sep 1, 2015·Chembiochem : a European Journal of Chemical Biology·Myles A MitchellKevin P C Minbiole
Nov 19, 2015·The Journal of Antibiotics·Maureen D JoyceKevin Pc Minbiole
Mar 31, 2016·ChemMedChem·Megan E FormanWilliam M Wuest
Jul 10, 2015·ACS Infectious Diseases·Megan C JenningsWilliam M Wuest
Jul 10, 2015·ACS Infectious Diseases·Megan C JenningsWilliam M Wuest
Citations
Oct 27, 2017·ChemMedChem·Stephanie A SchallenhammerKevin P C Minbiole
Dec 12, 2018·Applied and Environmental Microbiology·Sarah ForbesAndrew J McBain
May 23, 2019·ChemMedChem·Benoît ThomasMichel Boisbrun
Mar 4, 2020·Microorganisms·Urška RibičBarbara Jeršek
Dec 21, 2019·Chembiochem : a European Journal of Chemical Biology·Saleh AlkhalifaVincenzo Carnevale
May 31, 2018·MBio·Katherine HardyMark Webber
Jul 31, 2018·International Journal of Microbiology·Brice E N WambaVictor Kuete
Sep 5, 2020·ChemMedChem·Robert G CardenKevin P C Minbiole
Jan 12, 2021·World Journal of Microbiology & Biotechnology·Ewa ObłąkAnna Wieczyńska
Mar 23, 2021·IScience·Andrew R MahoneyAriel L Furst
Mar 24, 2021·ChemMedChem·Anna R KaplanWilliam M Wuest
Jun 3, 2021·Antibiotics·Angelica ArtasensiLaura Fumagalli
Nov 16, 2017·ACS Infectious Diseases·Kelly M Craft, Steven D Townsend
Mar 28, 2020·ACS Infectious Diseases·George M OngwaeMarcos M Pires
Nov 1, 2021·Journal of Industrial Microbiology & Biotechnology·Laura J FoxAndrew J McBain