EGb761 protects hydrogen peroxide-induced death of spinal cord neurons through inhibition of intracellular ROS production and modulation of apoptotic regulating genes.

Journal of Molecular Neuroscience : MN
Xiaoyan JiangXiao-Ming Xu

Abstract

The present study was conducted to investigate whether Ginkgo biloba extract (EGb) 761 could protect spinal cord neurons from H(2)O(2)-induced toxicity. In primary spinal cord neurons isolated from embryonic day 14 rats, H(2)O(2) administration resulted in a significant decrease in the survival of spinal cord neurons. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) and Hoechst 33342 nuclear staining showed that these cells die by apoptosis. Such neuronal death, however, was significantly reversed by EGb761 in a dose-dependent manner. Moreover, a marked increase in intracellular free radical generation was found after the H(2)O(2) administration which could be reversed almost completely by EGb761, indicating that inhibition of free radical generation is an important mechanism of the anti-apoptosis action of EGb761. Finally, treatment of cells with H(2)O(2) for 12 h reduced the expression of Bcl-2, an anti-apoptotic gene, by 70% but showed no effect on the level of Bax, a pro-apoptotic gene. EGb76 treatment, however, significantly reversed H(2)O(2)-induced reduction of Bcl-2 expression and inhibited Bax expression by 2.3-fold. Thus, our study provided evidence showing that the protective effect of EG...Continue Reading

References

Nov 7, 1992·Lancet·J Kleijnen, P Knipschild
May 1, 1988·Pharmacological Research Communications·H OberpichlerJ Krieglstein
Oct 22, 1993·Cell·D M HockenberyS J Korsmeyer
Nov 1, 1993·Trends in Neurosciences·C W Olanow
Jan 1, 1995·Methods in Cell Biology·M P MattsonR J Mark
Jan 1, 1994·Methods in Enzymology·L MarcocciM Gardès-Albert
Jun 15, 1994·Biochemical and Biophysical Research Communications·L MarcocciL Packer
Jan 20, 1997·The Journal of Comparative Neurology·P G PopovichB T Stokes
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·L H Boise, C B Thompson
Aug 15, 1997·Archives of Biochemistry and Biophysics·Y Y TyurinaV E Kagan
Jun 13, 1998·The Journal of Biological Chemistry·J Cai, D P Jones
Jul 29, 1998·Free Radical Biology & Medicine·D LiuJ Liu
Nov 21, 1998·Archives of Neurology·B S OkenJ A Kaye
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·D P MaxwellL McIntosh
Jul 30, 1999·Nature Medicine·J E SpringerP E Knapp
Dec 6, 2000·Progress in Brain Research·J R Bethea
Apr 5, 2002·Journal of Neuroscience Research·Alexander G RabchevskyStephen W Scheff
Oct 25, 2002·Cellular and Molecular Biology·Juan SastreJosé Viña
Dec 23, 2003·Cellular Signalling·Patricia RockwellEvan Gomes
Dec 23, 2003·Immunology Letters·Diomira LuongoMauro Rossi
Dec 31, 2003·Dental Materials : Official Publication of the Academy of Dental Materials·Y IssaA J Duxbury

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Citations

Aug 11, 2011·Neurochemistry International·Heba M MahdyAmani E Khalifa
Aug 29, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Norma Serrano-GarcíaCristina Trejo-Solís
Sep 8, 2018·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Kazuo TomitaTomoaki Sato
Jan 4, 2017·Reviews in the Neurosciences·Peng ZhangXun Ma
Feb 17, 2017·International Journal of Molecular Medicine·Jing-Xing ZhangJian-Guo Hu
Jan 10, 2017·International Journal of Molecular Sciences·Sai Wang SetoJianxun Liu
Jul 18, 2020·Oxidative Medicine and Cellular Longevity·Angélica Coyoy-SalgadoCarlos E Orozco-Barrios
May 28, 2011·Free Radical Research·Nilanjan GhoshSubhash C Mandal
May 1, 2021·Pharmaceuticals·Carlo TominoPaolo Maria Rossini

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