EGCG Protects against 6-OHDA-Induced Neurotoxicity in a Cell Culture Model

Parkinson's Disease
Dan ChenManju B Reddy

Abstract

Background. Parkinson's disease (PD) is a progressive neurodegenerative disease that causes severe brain dopamine depletion. Disruption of iron metabolism may be involved in the PD progression. Objective. To test the protective effect of (-)-epigallocatechin-3-gallate (EGCG) against 6-hydroxydopamine- (6-OHDA-) induced neurotoxicity by regulating iron metabolism in N27 cells. Methods. Protection by EGCG in N27 cells was assessed by SYTOX green assay, MTT, and caspase-3 activity. Iron regulatory gene and protein expression were measured by RT-PCR and Western blotting. Intracellular iron uptake was measured using (55)Fe. The EGCG protection was further tested in primary mesencephalic dopaminergic neurons by immunocytochemistry. Results. EGCG protected against 6-OHDA-induced cell toxicity. 6-OHDA treatment significantly (p < 0.05) increased divalent metal transporter-1 (DMT1) and hepcidin and decreased ferroportin 1 (Fpn1) level, whereas pretreatment with EGCG counteracted the effects. The increased (55)Fe (by 96%, p < 0.01) cell uptake confirmed the iron burden by 6-OHDA and was reduced by EGCG by 27% (p < 0.05), supporting the DMT1 results. Pretreatment with EGCG and 6-OHDA significantly increased (p < 0.0001) TH(+) cell count (...Continue Reading

References

Dec 1, 1989·Biochemical Pharmacology·H P Monteiro, C C Winterbourn
Jan 1, 1993·Movement Disorders : Official Journal of the Movement Disorder Society·M B YoudimP Riederer
Jun 26, 2001·Journal of Biochemical and Molecular Toxicology·N J Walker
Mar 11, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Orly WeinrebMoussa B H Youdim
Apr 28, 2004·Cell·Matthias W HentzeNancy C Andrews
Oct 16, 2004·Blood·Elizabeta NemethClara Camaschella
Dec 21, 2004·The Journal of Pharmacology and Experimental Therapeutics·Calivarathan LatchoumycandaneAnumantha G Kanthasamy
Mar 8, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xinchao WangAndrew J Ghio
Jun 18, 2005·The Journal of Biological Chemistry·Siddharth KaulAnumantha G Kanthasamy
Jul 20, 2006·Annual Review of Nutrition·Elizabeta Nemeth, Tomas Ganz
Oct 2, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Cindy CasteelsKoen Van Laere
Nov 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Julio SalazarEtienne C Hirsch
Feb 6, 2009·World Journal of Gastroenterology : WJG·Alberto PipernoDomenico Girelli
Jun 16, 2009·Current Opinion in Plant Biology·Robert Hänsch, Ralf R Mendel
Nov 10, 2009·Cellular and Molecular Life Sciences : CMLS·S-M WangY-Z Chang
Jul 16, 2010·Basic & Clinical Pharmacology & Toxicology·Hyo Geun KimMyung Sook Oh
Aug 24, 2010·Molecular and Cellular Biochemistry·Klaudia JomovaMarian Valko
Dec 17, 2010·World Journal of Hepatology·Duygu D Harrison-Findik
Feb 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Huajun JinAnumantha G Kanthasamy
Jun 19, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Hao-Yun ZhangJun-Xia Xie
Oct 25, 2013·Nutrients·Maria C Linder

❮ Previous
Next ❯

Citations

Sep 1, 2019·Medicinal Research Reviews·Zhong-Ming Qian, Ya Ke
Nov 26, 2016·Oxidative Medicine and Cellular Longevity·Haifeng LiZebo Huang
Oct 31, 2018·Frontiers in Neuroscience·Driton Vela
May 29, 2020·Neurochemical Research·Ashish SinghSarika Singh
Oct 27, 2018·Pharmaceuticals·Antonella RoettoRosa Maria Pellegrino

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

Prism
MetaMorph
Integrated Morphometry Analysis ( IMA )

Related Concepts

Related Feeds

Amygdala and Midbrain Dopamine

The midbrain dopamine system is widely studied for its involvement in emotional and motivational behavior. Some of these neurons receive information from the amygdala and project throughout the cortex. When the circuit and transmission of dopamine is disrupted symptoms may present. Here is the latest research on the amygdala and midbrain dopamine.