PMID: 9539953Apr 16, 1998Paper

EGF-induced programmed cell death of human mammary carcinoma MDA-MB-468 cells is preceded by activation AP-1

Cellular and Molecular Life Sciences : CMLS
P Schaerli, R Jaggi

Abstract

MDA-MB-468 is a human mammary adenocarcinoma cell line that overexpresses the epidermal growth factor (EGF) receptor and undergoes programmed cell death (apoptosis) in response to EGF treatment. Programmed cell death was shown to be greatly enhanced when cells were growth-arrested prior to EGF treatment. Apoptosis was characterized by an initial rounding up and detachment of the cells from their substrate starting about 12 h after EGF treatment, followed by chromatin condensation, nuclear fragmentation and oligonucleosomal fragmentation of the DNA at about 24 to 48 h. Cell death was dependent on de novo protein synthesis. We found a rapid induction of c-fos, c-jun and junB at the mRNA level after about 30 min of EGF treatment and a more delayed upregulation of fosB and fra-1. The junD gene was expressed in the absence of EGF, and it was moderately induced within 30 min of growth factor addition. The increase of the different fos and jun mRNAs were paralleled by an increase of activator protein-1 (AP-1) DNA binding activity. A characterization of the AP-1 complex revealed similar levels of several Fos and Jun proteins. Based on the kinetics of AP-1 accumulation and cell death, it seems likely that AP-1 contributes to the apoptot...Continue Reading

Citations

Apr 13, 2013·Nucleic Acids Research·Loredana PellegrinoLeandro Castellano
Jan 2, 2007·Annals of Surgical Oncology·Junichi KaganoiYoshiharu Sakai
Sep 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Zhong ZhaoDavid J Gross
Sep 27, 2008·Experimental Cell Research·Dustin C Hyatt, Brian P Ceresa
Dec 3, 2003·Cell Death and Differentiation·V AppiertoF Formelli
Feb 18, 2004·World Journal of Gastroenterology : WJG·Jane C J ChaoSheng-Hsuan Chen

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