EGF-induced trophoblast secretion of MMP-9 and TIMP-1 involves activation of both PI3K and MAPK signalling pathways

Reproduction : the Official Journal of the Society for the Study of Fertility
Qing QiuAndrée Gruslin

Abstract

Epidermal growth factor (EGF) is present in the maternal-fetal environment and has an important role in placental development. Matrix metalloproteinase-9 (MMP-9) expression/activation is a pre-requisite in extravillous trophoblast invasion. Whereas EGF up-regulates MMP-9 activity in a variety of cell types, there is no direct evidence for the stimulation of MMP-9 and tissue inhibitor of metalloproteinase-1 (TIMP-1) secretion by EGF in extravillous trophoblasts. In addition, the signalling pathways involved in this regulation are not clear. In the present study, we have examined the possible involvement of the phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinase (MAPK) pathways in the regulation of the MMP-9/TIMP-1 system by EGF in vitro. We used a well-established invasive extravillous trophoblast cell line (HTR8/Svneo) and measured gene and protein expression by semi-quantitative RT-PCR and western analysis respectively. MMP activity was determined by zymography. We showed for the first time that EGF activated both PI3K/Akt and MAPK/extracellular-signal regulated kinase (ERK) signalling in HTR8/SVneo, and increased both MMP-9 and TIMP-1 mRNAs and protein concentrations. Interfering with either signalling p...Continue Reading

References

Sep 1, 1996·European Journal of Obstetrics, Gynecology, and Reproductive Biology·M KolbenH Tschesche
Jan 1, 1997·Critical Reviews in Eukaryotic Gene Expression·P Borden, R A Heller
Dec 3, 1998·The Journal of Clinical Investigation·L GuedezM Stetler-Stevenson
Apr 20, 1999·Biochemical and Biophysical Research Communications·K NakataniR A Roth
Sep 7, 2000·The Journal of Clinical Investigation·B DuboisG Opdenakker
Jun 14, 2002·Journal of Reproductive Immunology·Paul BischofA Campana
Aug 30, 2002·Biochemical and Biophysical Research Communications·Pierre-Olivier EstèveYves St-Pierre
Jan 24, 2003·Critical Reviews in Biochemistry and Molecular Biology·Philippe E Van den SteenGhislain Opdenakker
Feb 5, 2003·Placenta·P BischofA Campana
Jun 6, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A R M Ruhul AminMichinari Hamaguchi

❮ Previous
Next ❯

Citations

Jun 26, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Gesa KreySandra M Blois
May 1, 2009·Histochemistry and Cell Biology·Baohua ZhangHongmei Wang
Oct 23, 2009·Histochemistry and Cell Biology·Xiao-jie MaYan-ling Wang
Jun 19, 2007·Apoptosis : an International Journal on Programmed Cell Death·Sarah J MollAlexander E P Heazell
Feb 19, 2009·Human Reproduction·James McCormickJudith E Cartwright
Feb 23, 2010·Molecular Human Reproduction·Rob D CatalanoHenry N Jabbour
Sep 29, 2011·Molecular and Cellular Biology·Lindsey N KentMichael J Soares
Nov 30, 2010·International Journal of Endocrinology·Emad KandilByron Crawford
Oct 21, 2005·Reproductive Biology and Endocrinology : RB&E·Elsebeth Staun-Ram, Eliezer Shalev
Sep 21, 2013·Disease Models & Mechanisms·Fenny WiradjajaIan Smyth
Jul 14, 2012·The Journal of Reproduction and Development·Michael J SoaresM A Karim Rumi
Oct 31, 2009·The International Journal of Developmental Biology·Martin Knöfler
May 9, 2012·The International Journal of Developmental Biology·Lindsey N KentMichael J Soares
Feb 11, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Ke-Ming XieDa-Jin Li
Oct 23, 2015·American Journal of Reproductive Immunology : AJRI·Satish Kumar GuptaPiyush Chaudhary
Apr 10, 2007·The Journal of Allergy and Clinical Immunology·Xiuxia ZhouSally E Wenzel
Jun 12, 2012·Toxicon : Official Journal of the International Society on Toxinology·Pei-Chien TsaiShinne-Ren Lin
Nov 20, 2013·Biochemical and Biophysical Research Communications·Michael J HerrLisa K Jennings
Jan 13, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ying-Hua ChangPao-Chi Liao

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.