EGF-like peptide-enhanced cell movement in Dictyostelium is mediated by protein kinases and the activity of several cytoskeletal proteins

Cellular Signalling
Robert J Huber, Danton H O'Day

Abstract

DdEGFL1, a synthetic epidermal growth factor-like (EGFL) peptide based on the first EGFL repeat of the extracellular matrix, cysteine-rich, calmodulin-binding protein CyrA, has previously been shown to sustain the threonine phosphorylation of a 210kDa protein during the starvation of Dictyostelium cells. Immunoprecipitation coupled with a LC/MS/MS analysis identified the 210kDa protein as vinculin B (VinB). VinB shares sequence similarity with mammalian vinculin, a protein that links the actin cytoskeleton to the plasma membrane. Both threonine phosphorylated VinB (P-VinB) and VinB-GFP localized to the cytoplasm and cytoskeleton of Dictyostelium amoebae. VinB-GFP was also shown to be threonine phosphorylated and co-immunoprecipitated with established vinculin-binding cytoskeletal proteins (e.g. myosin II heavy chain, actin, alpha-actinin, talin). P-VinB and VinB-GFP were detected in DdEGFL1 pull-down assays, which also identified a 135kDa phosphothreonine protein and two phosphotyrosine proteins (35 and 32kDa) as potential components of the DdEGFL1 signaling pathway. DdEGFL1-enhanced cell movement required the cytoskeletal proteins talin B and paxillin B and tyrosine kinase activity mediated by PKA signaling, however VinB threo...Continue Reading

References

Feb 1, 1992·The Journal of Cell Biology·D CoxD A Knecht
Oct 1, 1990·The Journal of Cell Biology·M BrinkM Schleicher
Jan 1, 1981·Differentiation; Research in Biological Diversity·B Varnum, D R Soll
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B GeigerS J Singer
Sep 1, 1994·The Journal of Cell Biology·A R MenkelB M Jockusch
Jul 27, 2001·The Journal of Cell Biology·C S SwindleA Wells
Jul 5, 2002·Nature·Gernot GlöcknerUNKNOWN Dictyostelium Genome Sequencing Consortium
Mar 22, 2003·Experimental Cell Research·Robert N JorissenAntony W Burgess
Jul 1, 2005·Molecular and Cellular Biology·Philippe R J BoisTina Izard
Jul 8, 2005·Molecular Biology of the Cell·Nathan D GallantAndrés J García
Feb 14, 2006·Developmental Biology·Jonathan R ChubbRobert H Singer
Aug 9, 2006·Trends in Cell Biology·Wolfgang H ZieglerDavid R Critchley
Feb 22, 2007·Journal of Cellular Physiology·Anand Krishnan V IyerAlan Wells
Mar 3, 2007·The Journal of Biological Chemistry·Kosuke TakedaRichard A Firtel
Apr 11, 2007·Developmental Cell·Lingfeng ChenPeter N Devreotes
Apr 28, 2007·Current Biology : CB·Oliver Hoeller, Robert R Kay
May 31, 2007·The Journal of Cell Biology·Peter J M van HaastertArjan Kortholt
Jan 25, 2008·Development·Christophe Anjard, William F Loomis
Apr 1, 2008·Eukaryotic Cell·Masatsune TsujiokaTaro Q P Uyeda
Dec 10, 2008·Plasmid·Douwe M VeltmanPeter J M Van Haastert
Jan 6, 2009·Biochemical and Biophysical Research Communications·Robert Huber, Danton H O'Day
Jan 10, 2009·Biochimica Et Biophysica Acta·Corinne GiustiPierre Golstein
Sep 8, 2009·Trends in Cell Biology·Jason S King, Robert H Insall
Aug 4, 2010·Biochemical and Biophysical Research Communications·Kevin KüpperBernd Hoffmann

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Citations

Jul 12, 2012·Cellular and Molecular Life Sciences : CMLS·Robert J Huber, Danton H O'Day
Aug 14, 2012·Biochemical and Biophysical Research Communications·Danton H O'DayAndres Suarez
Oct 23, 2016·Biochimica Et Biophysica Acta. General Subjects·Robert J Huber, Danton H O'Day
Nov 28, 2019·Biological Reviews of the Cambridge Philosophical Society·Danton H O'DayRobert J Huber

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