EGF Protects Cells Against Dox-Induced Growth Arrest Through Activating Cyclin D1 Expression

Journal of Cellular Biochemistry
Chun-Xia YaoLi-Xiang Xue

Abstract

It has been reported that the antitumor drug doxorubicin (Dox) exerts its toxic effects via GATA-4 depletion and that over-expression of GATA-4 reverses Dox-induced toxicity and apoptosis; however, the precise mechanisms remain unclear. In this study, we observed, for the first time, that EGF protects cells against Dox-mediated growth arrest, G2/M-phase arrest, and apoptosis. Additionally, EGF expression was down-regulated in Dox-treated cells and up-regulated in GATA-4 over-expressing cells. Utilizing real-time PCR and western blotting analysis, we found that the expression of the cell cycle-associated protein cyclin D1 was inhibited in GATA-4-silenced cells and Dox-treated cells and was enhanced in GATA-4 over-expressing cells and EGF-treated cells. Furthermore, EGF treatment reversed the inhibited expression of cyclin D1 that was mediated by GATA-4 RNAi or Dox. Our results indicate that EGF, as a downstream target of Dox, may be involved in Dox-induced toxicity as well as in the protective role of GATA-4 against toxicity induced by Dox via regulating cyclin D1 expression, which elucidates a new molecular mechanism of Dox toxicity with important clinical implications.

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Citations

Dec 24, 2018·Journal of Cellular Physiology·Si ZhangLixiang Xue
Aug 21, 2018·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yun Sun LeeSung Wook Seo
Apr 24, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ariadna Giró-PerafitaTeresa Puig

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