EGF receptor activation stimulates endogenous gastrin gene expression in canine G cells and human gastric cell cultures

The Journal of Clinical Investigation
M G FordJ L Merchant

Abstract

Gastrin release from the antral gastrin-expressing cell (G cell) is regulated by bombesin and luminal factors. Yet, these same extracellular regulators do not stimulate expression of the gene. Since the gastric mucosa expresses large quantities of EGF receptor ligands such as TGFalpha, we examined whether EGF receptor ligands stimulate gastrin gene expression in gastrin-expressing cell cultures. EGF receptor activation of primary cultures stimulated gastrin gene expression about twofold; whereas bombesin treatment of antral G cell cultures stimulated gastrin release but not gene expression. EGF and TGFalpha were weak stimulants of gastrin release. EGF receptor activation of AGS human gastric adenocarcinoma cell line stimulated gastrin gene expression nearly fourfold; and gastrin reporter constructs transfected into AGS cells were stimulated more than fourfold by EGF. EGF induction was conferred by the previously defined GC-rich gastrin EGF response element (gERE) element located at -68 to -53 bp upstream from the cap site since a mutation of the gERE element abolished both basal and EGF induction. Moreover, EGF treatment of AGS cells stimulated binding of the transcription factor Sp1 to this element. Collectively, these results...Continue Reading

References

Jan 1, 1992·Analytical Biochemistry·W P Deng, J A Nickoloff
Jan 1, 1990·The Journal of Clinical Investigation·J DelValle, T Yamada
Jun 19, 1991·Journal of the National Cancer Institute·S A WatsonD L Morris
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J M Godley, S J Brand
Mar 1, 1987·The Journal of Clinical Investigation·D H PolkD A Fisher
May 1, 1988·Molecular and Cellular Endocrinology·A PrzylipiakB Runnebaum
Nov 1, 1988·Peptides·R P ZimmermanP W Mantyh
Jun 1, 1972·European Journal of Clinical Investigation·H D Söling, K O Unger
Apr 1, 1984·Annals of Internal Medicine·R M Searcy, J R Malagelada
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L K JohnsonD Gospodarowicz
Nov 2, 1995·Biochemical and Biophysical Research Communications·T J Koh, T C Wang
May 1, 1995·Scandinavian Journal of Gastroenterology·L Vinter-JensenE Nexø
Feb 1, 1995·Journal of Cellular Physiology·G M HowellM G Brattain
Mar 17, 1995·The Journal of Biological Chemistry·J L MerchantD R Abraczinskas
Mar 12, 1993·Science·Y ShingJ Folkman

❮ Previous
Next ❯

Citations

May 12, 2000·Alimentary Pharmacology & Therapeutics·S SzaboM Yoshida
Oct 7, 2006·Current Opinion in Gastroenterology·P SinghA Owlia
May 19, 2000·Infection and Immunity·E Ofori-DarkoJ L Merchant
Dec 23, 2004·Journal of Virology·Elizabeth A Holley-GuthrieShannon C Kenney
Feb 22, 2001·Annual Review of Physiology·G J DockrayT Wang
Aug 30, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Edith Mensah-OsmanJuanita L Merchant
Nov 12, 2013·BMC Cancer·Catherine DoCatherine Seva
Jul 20, 2011·Gastroenterology·Daniel FourmyJean Claude Reubi
Jun 23, 2009·Molecular and Cellular Endocrinology·Jinghe LiuJulang Li
Feb 6, 2008·Alcohol·Vera TodorovićOlivera Mitrović
Nov 19, 2010·Helicobacter·Tamara P TuckerJuanita L Merchant
Jan 31, 2018·Frontiers in Cellular and Infection Microbiology·Niluka GunawardhanaJeong-Heon Cha
Nov 25, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Masako OgawaJames R Goldenring
Jan 17, 2003·American Journal of Physiology. Cell Physiology·Hua XuFayez K Ghishan
Feb 7, 2001·American Journal of Physiology. Cell Physiology·S ChupretaJ L Merchant
Jul 10, 2001·American Journal of Physiology. Cell Physiology·H XuF K Ghishan
Jun 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·X Wen, G D Wu
Feb 15, 2001·American Journal of Physiology. Cell Physiology·H XuF K Ghishan
Apr 2, 2002·World Journal of Gastroenterology : WJG·Feng-Peng SunYong-Li Yao
Nov 14, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·T SuzukiJ Del Valle
Jan 29, 2002·Biochemical and Biophysical Research Communications·Satoshi KusayanagiKeiji Mitamura
Jan 27, 1999·Biochemical and Biophysical Research Communications·J L MerchantA Todisco

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.