EGF stimulates proliferation in the bovine placental trophoblast cell line F3 via Ras and MAPK

Placenta
N HambruchC Pfarrer

Abstract

In the bovine placenta, multinucleate trophoblast giant cells (TGC), evolving from uninucleate trophoblast cells, are crucial for feto-maternal interaction as they show endocrine activity and the ability to migrate and fuse with caruncular epithelial cells. In contrast to caruncular epithelial cells, the isolation and culture of bovine trophoblast cells is complicated because they cease to express their specific products, like placental lactogen (PL), during prolonged culture. In the present study, we aimed to establish a bovine cotyledonary trophoblast cell line targeting our long term goal to develop an in vitro model for the bovine placenta. Therefore, the functional activity of important signalling pathways was tested. Primary trophoblast cells were isolated from a bovine cotyledon of a male fetus and successfully subcultured and cryopreserved. The obtained cell line, termed F3, showed epithelial morphology and characteristic binuclear giant cells in small numbers through all passages. The trophoblastic origin of F3 cells was verified by amplification of a Y-chromosome specific DNA-sequence and the presence of PL mRNA. Immunofluorescence demonstrated that F3 cells were continuously positive for zonula occludens-2 (ZO-2), cy...Continue Reading

References

Oct 11, 1991·The Journal of Clinical Endocrinology and Metabolism·G E HofmannL Deligdisch
Oct 1, 1994·The Journal of Pediatric Endocrinology·D Evain-Brion, E Alsat
Jul 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T M DuelloP J Van Ess
Feb 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J MühlhauserM Castellucci
Nov 20, 1998·Molecular Reproduction and Development·A KliemB Fischer
Jul 15, 1999·The International Journal of Biochemistry & Cell Biology·A Wells
Oct 21, 1999·Biology of Reproduction·G A JohnsonT E Spencer
Nov 30, 1999·Trends in Pharmacological Sciences·E ZwickA Ullrich
Jul 5, 2005·Placenta·E D JohnstoneL J Guilbert
Feb 28, 2006·International Journal for Parasitology·Carlos HermosillaAnja Taubert
May 24, 2006·Animal Reproduction Science·L P LandimJ M Garcia
Mar 25, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Kidsadagon PringproaKonstantin Wewetzer

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Citations

Apr 1, 2014·Journal of Assisted Reproduction and Genetics·V A Absalón-MedinaR O Gilbert
Aug 25, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Yasunori SuzukiKazuyoshi Hashizume
Feb 12, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Qi En YangAlan D Ealy
Oct 27, 2010·Cells, Tissues, Organs·J D HaegerC Pfarrer
Nov 22, 2011·Molecular Reproduction and Development·Hanako BaiKazuhiko Imakawa
Nov 27, 2010·Biochemical and Biophysical Research Communications·Maren IlowskiWolfgang E Thasler
May 9, 2016·Theriogenology·J-D HaegerC Pfarrer
May 24, 2014·Biology of Reproduction·Priscila Ramos-IbeasMiguel Ángel Ramírez
May 19, 2012·Vascular and Endovascular Surgery·Li LiLiNa Yang
Feb 3, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rulan BaiKazuhiko Imakawa
Nov 27, 2019·Nature Ecology & Evolution· KshitizGünter P Wagner
Feb 14, 2021·Reproduction in Domestic Animals = Zuchthygiene·Monika JamiołMarta Kankofer
Feb 9, 2021·Frontiers in Veterinary Science·Iván Pastor-FernándezPilar Horcajo

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