EGFR Mutations in Cell-free Plasma DNA from Patients with Advanced Lung Adenocarcinoma: Improved Detection by Droplet Digital PCR

Targeted Oncology
Anna BuderMartin Filipits

Abstract

Analysis of cell-free DNA from blood could provide an alternative method for identifying genomic changes in the tumors of patients with advanced lung adenocarcinoma. We compared the performance of droplet digital PCR (ddPCR) and Cobas® EGFR Mutation Test v2 (Cobas) for detecting EGFR mutations in cell-free plasma DNA. Plasma samples from patients with advanced EGFR-mutated lung adenocarcinoma were analyzed for EGFR T790M, exon 19 deletions, and L858R mutations by both ddPCR and Cobas. T790M testing was performed in 354 plasma samples collected from 129 patients. The concordance rate between ddPCR and Cobas for T790M, sensitivity, and specificity were 86, 100, and 85%, respectively. Exon 19 deletions were analyzed in 196 plasma samples obtained from 71 of the 129 patients using both platforms. The concordance rate between ddPCR and Cobas for exon 19 deletions, sensitivity, and specificity were 90, 92, and 89%, respectively. L858R mutations were studied in 124 plasma samples obtained from 44 of the 129 patients using both assays. The concordance rate between ddPCR and Cobas for L858R, sensitivity, and specificity were 90, 91, and 89%, respectively. In patients who progressed under treatment with an EGFR TKI (n = 50), the T790M po...Continue Reading

References

Jan 17, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Geoffrey R OxnardPasi A Jänne
Oct 9, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tilak K SundaresanDaniel A Haber
Oct 24, 2015·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Kenneth S ThressJ Carl Barrett
Jan 6, 2016·Current Opinion in Oncology·Anna BuderMartin Filipits
Feb 4, 2016·Future Oncology·Paul Brown
Jul 30, 2016·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Karen L ReckampShirish Gadgeel
Dec 14, 2016·The New England Journal of Medicine·Tony S MokUNKNOWN AURA3 Investigators
Nov 21, 2017·The New England Journal of Medicine·Jean-Charles SoriaUNKNOWN FLAURA Investigators
Mar 6, 2018·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Anna BuderMartin Filipits

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Citations

Dec 12, 2020·Expert Review of Molecular Diagnostics·Andrea Moreno-ManuelEloisa Jantus-Lewintre
Mar 2, 2021·Clinical Medicine Insights. Oncology·Klaus HacknerMartin Filipits
Apr 27, 2021·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Robert PirkerMartin Filipits

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Methods Mentioned

BETA
PCR
biopsy
genotyping

Software Mentioned

Cobas
QuantaSoft
SPSS

Related Concepts