EglC, a new endoglucanase from Aspergillus niger with major activity towards xyloglucan.

Applied and Environmental Microbiology
A A HasperL H de Graaff

Abstract

A novel gene, eglC, encoding an endoglucanase, was cloned from Aspergillus niger. Transcription of eglC is regulated by XlnR, a transcriptional activator that controls the degradation of polysaccharides in plant cell walls. EglC is an 858-amino-acid protein and contains a conserved C-terminal cellulose-binding domain. EglC can be classified in glycoside hydrolase family 74. No homology to any of the endoglucanases from Trichoderma reesei was found. In the plant cell wall xyloglucan is closely linked to cellulose fibrils. We hypothesize that the EglC cellulose-binding domain anchors the enzyme to the cellulose chains while it is cleaving the xyloglucan backbone. By this action it may contribute to the degradation of the plant cell wall structure together with other enzymes, including hemicellulases and cellulases. EglC is most active towards xyloglucan and therefore is functionally different from the other two endoglucanases from A. niger, EglA and EglB, which exhibit the greatest activity towards beta-glucan. Although the mode of action of EglC is not known, this enzyme represents a new enzyme function involved in plant cell wall polysaccharide degradation by A. niger.

References

May 1, 1994·Molecular Microbiology·L H de GraaffJ Visser
Mar 13, 1997·Carbohydrate Research·J P VinckenA G Voragen
Oct 6, 1998·Applied and Environmental Microbiology·N N van PeijL H de Graaff
Jan 1, 1953·Advances in Genetics·G PONTECORVOA W J BUFTON

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Citations

Mar 8, 2012·Journal of Industrial Microbiology & Biotechnology·Ho Myeong KimHyeun-Jong Bae
Jul 9, 2003·European Journal of Biochemistry·Diana C IrwinDavid B Wilson
Mar 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mikael R AndersenJens Nielsen
Nov 22, 2011·Applied and Environmental Microbiology·Bolormaa EnkhbaatarSoon-Kwang Hong
Feb 22, 2012·Applied and Environmental Microbiology·Astrid R Mach-AignerLeo H de Graaff
Dec 8, 2005·Applied and Environmental Microbiology·Katsuro YaoiYasushi Mitsuishi
Jul 18, 2012·BMC Genomics·Mikael R AndersenJens Nielsen
Oct 1, 2013·BMC Genomics·Aleksandrina PatyshakuliyevaRonald P de Vries
Jun 28, 2011·BMC Systems Biology·Jimmy OmonyAnton J B van Boxtel
Jul 26, 2011·Applied Microbiology and Biotechnology·Joost van den Brink, Ronald P de Vries
Sep 28, 2014·Journal of Industrial Microbiology & Biotechnology·Helena CulletonRonald P de Vries
Mar 25, 2014·FEBS Letters·Tomohiko MatsuzawaKatsuro Yaoi
Feb 24, 2016·Biotechnology for Biofuels·Maria Victoria Aguilar-PontesJoost van den Brink
Jul 15, 2015·Applied Microbiology and Biotechnology·Vanessa O A PellegriniIgor Polikarpov
Jan 15, 2010·Plant Biotechnology Journal·Dheeraj VermaHenry Daniell
Feb 7, 2015·Biotechnology Letters·Ankita Shrivastava BhattacharyaBrett I Pletschke
Mar 13, 2014·Revista iberoamericana de micología·Ivonne Gutiérrez-RojasDolly Montoya
May 8, 2016·Biochemical and Biophysical Research Communications·Junjie YanRey-Ting Guo
Jun 2, 2016·Applied Microbiology and Biotechnology·Gabriela Leal VitcosqueMaria de Lourdes T M Polizeli
Aug 16, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chien-Huang LiTsong-Rong Yan
Nov 25, 2017·BMC Genomics·Birgit S GrubenRonald P De Vries
Feb 10, 2018·Folia Microbiologica·Christian HernándezEnrique Alarcón
Jun 16, 2017·Biotechnology for Biofuels·Tiziano BenocciRonald P de Vries
May 11, 2004·Microbiology·Alinda A HasperLeo H de Graaff
Sep 11, 2020·Applied Microbiology and Biotechnology·Tomohiko MatsuzawaKatsuro Yaoi
Jun 1, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Evandro Ares de AraújoIgor Polikarpov
Jul 22, 2019·The Journal of Biological Chemistry·Gregory ArnalHarry Brumer

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