EGR-1, a UV-inducible gene in p53(-/-) mouse cells

Experimental Cell Research
W Zhang, S Chen

Abstract

Changes in gene expression were examined in p53(-/-) and p53(+/+) mouse cells after ultraviolet (UV) irradiation. Differential display was used to identify differentially expressed gene(s) in UV-treated p53(-/-) and p53(+/+) cells. One of the differentially expressed genes was EGR-1 (early growth response gene-1), which was shown to be induced only in p53(-/-) cells. The induction of this gene by UV was detected as early as 0.5 h, peaked at 2 h, and returned to normal levels by 4 h. De novo protein synthesis was not required for UV-induced EGR-1 expression in p53(-/-) cells. Pretreatment of p53(-/-) cells with suramin, an inhibitor of growth factor receptors, completely suppressed UV-induced EGR-1 expression, suggesting that the induction may be mediated via the growth factor receptors. The presence of wild-type p53 suppressed the induction of EGR-1 after UV treatment. Overexpression of EGR-1 promoted the UV-induced transformation in p53(+/+) cells, but not in p53(-/-) cells. These data suggested that EGR-1 may be an important player in the UV responses of mammalian cells and may influence UV-induced transformation.

References

Jul 1, 1991·Molecular and Cellular Biology·K FukasawaS Chen
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·U SanthanamP B Sehgal
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D E HallahanR R Weichselbaum
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D GinsbergM Oren
Dec 20, 1990·Nature·B Vogelstein
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C BetsholtzB Westermark
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P LemaireP Charnay
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B A ChristyD Nathans
Sep 1, 1984·Molecular and Cellular Biology·W Maltzman, L Czyzyk
Jun 1, 1995·Nature Genetics·V P SahT Jacks
Sep 23, 1994·Cell·C SachsenmaierH J Rahmsdorf
Nov 19, 1993·Cell·W S el-DeiryB Vogelstein
Feb 5, 1998·Carcinogenesis·W ZhangS Chen
Mar 11, 1999·Genes & Development·M SchreiberE F Wagner

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Citations

Jan 20, 2004·Molecular and Cellular Biology·Koji NakadeBohdan Wasylyk
Sep 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Shanthi Adimoolam, James M Ford
Jun 1, 2014·Archives of Pathology & Laboratory Medicine·Stefan Kraft, Scott R Granter
Nov 22, 2005·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Olaug Kristin RødningenAnne-Lise Børresen-Dale
Feb 5, 2003·Biochemical Pharmacology·Eric Van Den NesteFrançoise Bontemps
Nov 15, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Courtney W HouchenWilliam F Stenson
Oct 9, 2015·Journal of Cell Science·Sandra SchickVijay K Tiwari
Jul 14, 2021·The Journal of Pharmacology and Experimental Therapeutics·Ya-Jie XiongZhong-Qin Liang

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