Egr-1 mediated cardiac miR-99 family expression diverges physiological hypertrophy from pathological hypertrophy.

Experimental Cell Research
Subbiah RamasamyRajan Sudarsan

Abstract

The physiological cardiac hypertrophy is an adaptive condition without myocyte cell death, while pathological hypertrophy is a maladaptive condition associated with myocyte cell death. This study explores the miRNome of α-2M-induced physiologically hypertrophied cardiomyocytes and the role of miRNA-99 family during cardiac hypertrophy. Physiological and pathological cardiac hypertrophy was induced in H9c2 cardiomyoblast cell lines using α-2M and isoproterenol respectively. Total RNA isolation and small RNA sequencing were executed for physiological hypertrophy model. The differentially expressed miRNAs and its target mRNAs were validated in animal models. Transcription factor binding sites were predicted in the promoter of specific miRNAs and validated by ChIP-PCR. Subsequently, the selected miRNA was functionally characterized by overexpression and silencing. The effects of silencing of upstream regulator and downstream target gene were studied. Analysis of small RNA reads revealed the differential expression of a large set of miRNAs during hypertrophy, of which miR-99 family was highly downregulated upon α-2M treatment. However, this miR-99 family expression was upregulated during pathological hypertrophy and confirmed in ani...Continue Reading

Citations

Jan 28, 2020·Circulation·Clarissa Pedrosa Costa GomesUNKNOWN EU-CardioRNA COST Action (CA17129)
Jun 28, 2020·Apoptosis : an International Journal on Programmed Cell Death·Anusha SivakumarRekha Balakrishnan
Dec 7, 2020·Journal of Molecular and Cellular Cardiology·K Shanmugha RajanVemparthan Suvekbala
Jul 11, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Nahid AlimoradiReihaneh Fatehi
Jul 9, 2021·Environmental Toxicology and Pharmacology·Sasikumar SundaresanGovindan Sadasivam Selvam
Oct 5, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christian SmolkaFranziska Pankratz
Dec 28, 2021·European Journal of Heart Failure·Kirsten T NijholtB Daan Westenbrink

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