Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein.

Virology Journal
Lei WangQihan Li

Abstract

HSV-1 viral capsid maturation and egress from the nucleus constitutes a self-controlled process of interactions between host cytoplasmic membrane proteins and viral capsid proteins. In this study, a member of the tetraspanin superfamily, CTMP-7, was shown to physically interact with HSV-1 protein VP26, and the VP26-CTMP-7 complex was detected both in vivo and in vitro. The interaction of VP26 with CTMP-7 plays an essential role in normal HSV-1 replication. Additionally, analysis of a recombinant virus HSV-1-UG showed that mutating VP26 resulted in a decreased viral replication rate and in aggregation of viral mutant capsids in the nucleus. Together, our data support the notion that biological events mediated by a VP26 - CTMP-7 interaction aid in viral capsid enveloping and egress from the cell during the HSV-1 infectious process.

References

Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z H ZhouF J Rixon
May 8, 2000·Science·Z H ZhouW Chiu
Mar 27, 2001·Journal of Virology·H GranzowT C Mettenleiter
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P J CozziY Fong
Jan 19, 2002·Journal of Virology·Thomas C Mettenleiter
Nov 25, 2003·Science·Kay GrünewaldAlasdair C Steven
Nov 30, 2004·Virus Research·Thomas C Mettenleiter
Aug 17, 2005·Journal of Virology·F MartinG W Moseley
Sep 29, 2005·Journal of Virology·Helene LeuzingerPeter Wild
Nov 30, 2005·Nature Reviews. Molecular Cell Biology·Martin E Hemler
Oct 19, 2007·Journal of Virology·Sonia MolinaChantal Fournier-Wirth
Mar 14, 2008·Journal of Virology·Anna Paula de OliveiraCornel Fraefel
Jun 19, 2008·Journal of Cell Science·Ivan LiashkovichVictor Shahin

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Citations

Oct 14, 2011·Viruses·Sofia V ZaichickGregory A Smith
Nov 28, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Aline de OliveiraTin-Chun Chu
May 7, 2014·Biochimica Et Biophysica Acta·Patrik BjörkholmGunnar von Heijne
Mar 12, 2015·Proteomics·Daniell L RowlesIleana M Cristea
Dec 21, 2018·Journal of Virology·Michael P HantakTom Gallagher
Jun 6, 2020·Medical Microbiology and Immunology·Beatriz BenayasMaría Yáñez-Mó
Jun 12, 2018·Frontiers in Immunology·Luise Florin, Thorsten Lang

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Methods Mentioned

BETA
two-hybrid
co-immunoprecipitation
transfection
fluorescence microscopy
flow cytometry
density gradient centrifugation
PCR
electron microscopy
electrophoresis

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