Egress of light particles among filopodia on the surface of Varicella-Zoster virus-infected cells

Journal of Virology
John E CarpenterCharles Grose

Abstract

Varicella-zoster virus (VZV) is renowned for its very low titer when grown in cultured cells. There remains no single explanation for the low infectivity. In this study, viral particles on the surfaces of infected cells were examined by several imaging technologies. Few surface particles were detected at 48 h postinfection (hpi), but numerous particles were observed at 72 and 96 hpi. At 72 hpi, 75% of the particles resembled light (L) particles, i.e., envelopes without capsids. By 96 hpi, 85% of all particles resembled L particles. Subsequently, the envelopes of complete virions and L particles were investigated to determine their glycoprotein constituents. Glycoproteins gE, gI, and gB were detected in the envelopes of both types of particles in similar numbers; i.e., there appeared to be no difference in the glycoprotein content of the L particles. The viral particles emerged onto the cell surface amid actin-based filopodia, which were present in abundance within viral highways. Viral particles were easily detected at the base of and along the exterior surfaces of the filopodia. VZV particles were not detected within filopodia. In short, these results demonstrate that VZV infection of cultured cells produces a larger proportio...Continue Reading

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Citations

May 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Céline Van den BroekeHerman W Favoreel
Apr 17, 2009·Journal of Virology·John E CarpenterCharles Grose
Mar 20, 2009·Journal of Virology·Marie-Noëlle TakahashiLuca Benetti
Feb 18, 2010·Future Virology·D Curanovic, Lw Enquist
Mar 26, 2016·Frontiers in Microbiology·Kenneth ChangVaibhav Tiwari
Sep 27, 2014·The Journal of Pathology·Werner Jd Ouwendijk, Georges Mgm Verjans
Mar 4, 2010·Veterinary Microbiology·Herman W FavoreelNick De Regge
Aug 30, 2012·Nucleus·Oren KobilerAriella Oppenheim
Mar 4, 2010·Veterinary Microbiology·Agnieszka BrzozowskaKrystyna Bieńkowska-Szewczyk
Feb 3, 2015·Trends in Microbiology·Matthew P Taylor, Lynn W Enquist
Feb 14, 2018·Viruses·Christopher E DenesRussell J Diefenbach

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