EH domain-dependent interactions between Eps15 and clathrin-coated vesicle protein p95

Biochemical and Biophysical Research Communications
P S McPhersonS E Egan

Abstract

The endocytic protein Eps15 contains three copies of the EH domain, a protein module thought to function in protein-protein interactions. Using overlay assays with an Eps15 EH domain fusion protein, we have now identified a protein of 95 kDa (p95) as a major EH domain-binding partner in a wide variety of tissues. The amino acids asparagine-proline-phenylalanine (NPF) form the core of an EH domain-binding motif and three NPF repeats are found in the endocytic protein synaptojanin-170. We have confirmed previous studies indicating that synaptojanin-170 is an EH domain-binding protein, and have used peptide blocking experiments to demonstrate that the interaction is mediated through the NPF repeats. Interestingly, the same peptide also blocks EH domain-binding to p95. Finally, we have shown that p95 is enriched on clathrin-coated vesicles, suggesting an endocytic role for the protein. These data support an important role for EH domain-NPF motif interactions in endocytosis.

References

Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·W T WongP P Di Fiore
Sep 1, 1993·Molecular and Cellular Biology·F FazioliP P Di Fiore
Jun 23, 1995·The Journal of Biological Chemistry·C SchumacherH Hanafusa
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·P S McPhersonP De Camilli
Jan 25, 1996·Nature·P S McPhersonP De Camilli
Dec 1, 1995·The Journal of Cell Biology·A BenmerahN Cerf-Bensussan
May 17, 1996·The Journal of Biological Chemistry·A BenmerahN Cerf-Bensussan
Nov 15, 1996·The Journal of Biological Chemistry·F TebarT Kirchhausen
Mar 28, 1997·The Journal of Biological Chemistry·E de HeuvelP S McPherson
May 1, 1997·Current Biology : CB·P T StukenbergM W Kirschner
Jun 13, 1997·The Journal of Biological Chemistry·F TebarA Sorkin
Oct 27, 1997·The Journal of Biological Chemistry·K D MichevaP S McPherson
Jan 10, 1998·The Journal of Biological Chemistry·Y NemotoP DeCamilli

❮ Previous
Next ❯

Citations

Sep 28, 2002·Nature·Marijn G J FordHarvey T McMahon
Dec 18, 2003·Science's STKE : Signal Transduction Knowledge Environment·Jonathan P DiNittoDavid G Lambright
Dec 28, 1999·Annual Review of Cell and Developmental Biology·M J HannahW B Huttner
Aug 28, 2012·Journal of Molecular Biology·Chun-Liang LaiGregory A Voth
Dec 2, 1999·Experimental Cell Research·E SantoliniP P Di Fiore
Feb 18, 1999·Current Biology : CB·B J Mayer
Mar 26, 2002·FEBS Letters·Pietro De CamilliAxel T Brunger
Dec 17, 2003·FEBS Letters·Sylwia WasiakPeter S McPherson
May 22, 2001·The Journal of Biological Chemistry·K D SpradlingB K Pilcher
Apr 15, 2000·The Journal of Biological Chemistry·K KariyaA Kikuchi
Nov 13, 1998·The Journal of Biological Chemistry·M YamabhaiB K Kay

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.