EHD2 regulates adipocyte function and is enriched at cell surface-associated lipid droplets in primary human adipocytes

Molecular Biology of the Cell
Björn MorénKarin G Stenkula

Abstract

Adipocytes play a central role in energy balance, and dysfunctional adipose tissue severely affects systemic energy homeostasis. The ATPase EH domain-containing 2 (EHD2) has previously been shown to regulate caveolae, plasma membrane-specific domains that are involved in lipid uptake and signal transduction. Here, we investigated the role of EHD2 in adipocyte function. We demonstrate that EHD2 protein expression is highly up-regulated at the onset of triglyceride accumulation during adipocyte differentiation. Small interfering RNA-mediated EHD2 silencing affected the differentiation process and impaired insulin sensitivity, lipid storage capacity, and lipolysis. Fluorescence imaging revealed localization of EHD2 to caveolae, close to cell surface-associated lipid droplets in primary human adipocytes. These lipid droplets stained positive for glycerol transporter aquaporin 7 and phosphorylated perilipin-1 following adrenergic stimulation. Further, EHD2 overexpression in human adipocytes increased the lipolytic signaling and suppressed the activity of transcription factor PPARγ. Overall, these data suggest that EHD2 plays a key role for adipocyte function.

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Citations

Feb 13, 2020·Biochemical Society Transactions·Madlen HubertRichard Lundmark
May 13, 2020·Journal of Cell Science·Albert PolRobert G Parton
Mar 15, 2020·Proceedings of the National Academy of Sciences of the United States of America·Claudia MatthaeusOliver Daumke
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Mar 12, 2021·Frontiers in Cell and Developmental Biology·Claudia Matthaeus, Justin W Taraska

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Datasets Mentioned

BETA
CU013410
BC113161

Methods Mentioned

BETA
confocal microscopy
Fluorescence
FCS
transfection

Software Mentioned

GraphPad Prism
NIS
Elements

Related Concepts

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Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.

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