Abstract
Phospholipase A(2) (PLA(2)) catalyzes hydrolysis of phospholipids at sn-2 position and usually releases arachidonic acid, which is oxygenated into various eicosanoids that mediate innate immune responses in insects. PLA(2) activities were measured in both immune-associated tissues of hemocyte and fat body in the beet armyworm, Spodoptera exigua. Upon challenge of an entomopathogenic fungus, Beauveria bassiana, the PLA(2)s were significantly activated in both hemocyte and fat body. The fungal infection also induced gene expression of antimicrobial peptides (AMPs), such as two attacins, cecropin, gallerimycin, gloverin, hemolin, and transferrin of S. exigua. RNA interference of Toll or Imd signal pathway using double-stranded RNAs (dsRNAs) specific to SeToll or SeRelish suppressed specific AMP gene expressions, in which dsRNA specific to SeToll suppressed two attacins, cecropin, gallerimycin, gloverin, hemolin, and transferrin I, while dsRNA specific to SeRelish suppressed only cecropin. Interestingly, dsRNA specific to SeToll also significantly inhibited the activation of PLA(2) in response to the fungal infection, but dsRNA specific to SeRelish did not. Eicosanoid-dependent hemocyte nodulation was inhibited by dsRNA specific to...Continue Reading
References
Jun 14, 1991·Cell·J D ClarkJ L Knopf
Feb 15, 1989·Analytical Biochemistry·F RadvanyiC Bon
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·J S MillerD W Stanley-Samuelson
Nov 1, 1996·The Journal of Biological Chemistry·R M KramerJ A Jakubowski
Nov 18, 1997·Cell·R Medzhitov, C A Janeway
Jan 13, 1998·FEBS Letters·I MorishimaN Matsuo
Jul 13, 2000·Journal of Insect Physiology·Y Park, Y Kim
Dec 14, 2001·Nature·T MichelJ Royet
Jun 11, 2002·The Journal of Parasitology·Yves CartonAnthony J Nappi
Jul 4, 2002·Journal of Invertebrate Pathology·Paul DeanStuart E Reynolds
Jan 7, 2003·The Biochemical Journal·Masashi YajimaShoichiro Kurata
Feb 19, 1997·Journal of Insect Physiology·R G.H. DownerC A. Mandato
Nov 7, 2003·Nature·Jules A Hoffmann
Jun 18, 2004·Immunological Reviews·Vincent Leclerc, Jean-Marc Reichhart
Oct 13, 2004·FEMS Microbiology Letters·Dongjin JiYonggyun Kim
Feb 22, 2005·The Journal of Biological Chemistry·Sang Woon ShinAlexander S Raikhel
Mar 31, 2005·Trends in Immunology·Takahiro Tanji, Y Tony Ip
Sep 24, 2005·The Journal of Biological Chemistry·Hai-Yan Qi, James H Shelhamer
Dec 13, 2005·Critical Care Medicine·James A Cook
Oct 19, 2006·Archives of Insect Biochemistry and Physiology·Hasan Tunaz
Jan 5, 2007·Annual Review of Immunology·Bruno Lemaitre, Jules Hoffmann
Mar 31, 2007·Journal of Invertebrate Pathology·Sony Shrestha, Yonggyun Kim
Aug 31, 2007·Genome Biology·Zhen ZouHaobo Jiang
Dec 12, 2007·Insect Biochemistry and Molecular Biology·Sony Shrestha, Yonggyun Kim
Oct 7, 2008·Insect Biochemistry and Molecular Biology·Hiromitsu TanakaMinoru Yamakawa
Sep 8, 2009·Bioscience, Biotechnology, and Biochemistry·Sony Shrestha, Yonggyun Kim
Nov 26, 2009·Journal of Insect Physiology·Sony ShresthaYonggyun Kim
Jan 2, 2010·Developmental and Comparative Immunology·Sony Shrestha, Yonggyun Kim
Jul 6, 2010·Journal of Invertebrate Pathology·Bokri Park, Yonggyun Kim
Citations
Oct 1, 2013·Journal of Invertebrate Pathology·Qiu-Ning LiuChao-Liang Liu
May 8, 2014·PloS One·Liang GongQiongbo Hu
Dec 3, 2014·Insect Biochemistry and Molecular Biology·Youshan LiQingyou Xia
Dec 21, 2014·Gene·Cong-Fen ZhangChao-Liang Liu
Sep 7, 2016·Parasites & Vectors·Mariana M ChavesRobson Coutinho-Silva
Dec 8, 2016·Insect Science·Elżbieta CzarniewskaMariola Kuczer
Aug 19, 2018·Archives of Insect Biochemistry and Physiology·Hyoil KimYonggyun Kim
Jan 1, 2012·Insects·Kent S Shelby, Holly J R Popham
Apr 12, 2017·The Journal of Parasitology·Reyhaneh DarsoueiMojtaba Hosseini
Aug 8, 2020·Insects·Md Mahi Imam MollahYonggyun Kim
Sep 1, 2013·Insects·Ángel M Casanova-Torres, Heidi Goodrich-Blair
Dec 4, 2019·Insect Biochemistry and Molecular Biology·Hyeogsun KwonPatricia V Pietrantonio